Abcam, ab34759, Anti-Hantavirus nucleocapsid protein antibody [5E11]

Size: 1mL
Mouse Monoclonal NCAP antibody. Suitable for ICC/IF and reacts with Hantavirus Puumala strain samples. Cited in 4 publications.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:5E11,
Isotype:IgG1,
Carrier free:No,
Reacts with:Hantavirus Puumala strain,
Applications:ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Epitope:The epitope recognized by this antibody is localized at the N-terminus, between amino acids 1 and 45 of hantavirus nucleocapsid protein.,
Specificity:This antibody reacts with nucleocapsid proteins of Puumala hantaviruses: Vranica, Sotkamo and Kazan and reacts with hantavirus-infected cells. It cross-reacts with nucleocapsid proteins of Andes, Sin Nombre and Seoul hantaviruses. It does not cross-react with hamster polyomavirus major capsid protein VP1, nor with nucleocapsid proteins of measles and mumps viruses.

Product details:
This product was changed from ascites to tissue culture supernatant on 28/11/2017. Please note that the dilutions may need to be adjusted accordingly.

Properties and Storage Information:
Form-Liquid, Purity-Tissue culture supernatant, Storage buffer-pH: 6.8 - 7.2Preservative: 0.1% Sodium azideConstituents: Tissue culture supernatant, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Hantavirus nucleocapsid protein also referred to as the N protein is an essential structural component of the hantavirus. It plays a mechanical role in packaging the viral RNA genome into ribonucleoprotein complexes during virus assembly. The nucleocapsid protein has a mass of around 50 kDa and exhibits a high level of conservation across different hantavirus strains. Expression of this protein occurs in infected host cells where it facilitates virus replication and assembly.
Biological function summary
The hantavirus nucleocapsid protein associates with the RNA genome to form a nucleocapsid complex critical for viral replication. This protein aids in the encapsidation of viral RNA ensuring proper genome replication and transcription. It also interacts with viral polymerase and other cellular factors to modulate gene expression and replication processes. The nucleocapsid protein is expressed within the cytoplasm of host cells where it orchestrates essential steps in the hantavirus life cycle.
Pathways
The hantavirus nucleocapsid protein participates in the viral replication and transcription pathway. This protein interacts with the L protein the viral RNA-dependent RNA polymerase to enhance viral replication efficiency. The protein also plays a part in the cellular stress response pathway due to its evasion of host cell immune responses. These interactions emphasize the nucleocapsid protein’s central role in ensuring effective hantavirus assembly and replication.
Hantavirus nucleocapsid protein has an important role in diseases such as Hantavirus Pulmonary Syndrome (HPS) and Hemorrhagic Fever with Renal Syndrome (HFRS). These conditions arise from the viral infection mediated by the nucleocapsid protein's functions. The protein has significant interactions with host cell proteins that alter immune responses contributing to disease pathogenesis. Understanding its mechanistic and biological functions is essential for developing therapies or preventative measures against hantavirus-related diseases.