Abcam, ab3572, Anti-Cytochrome P450 3A4/CYP3A4 antibody

Size: 100µL
Rabbit Polyclonal Cytochrome P450 3A4/CYP3A4 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 39 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CYP3A4.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human CYP3A4.P08684,
Specificity:ab3572 detects cytochrome P450 (CYP) 3A4 in human and rat tissues and cells. It also shows cross-reactivity with other P450 isoforms to a lesser extent. Since mice do not express CYP3A4, the bands observed in mouse western blot samples correspond to CYP3A9 and CYP3A1.

Properties and Storage Information:
Form-Liquid, Purification technique-Precipitation Ammonium Sulphate, Storage buffer-Preservative: 0.05% Sodium azideConstituents: PBS, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Cytochrome P450 3A4 also known as CYP3A4 is an enzyme that plays an important role in the metabolism of many xenobiotics and endogenous compounds. It belongs to the cytochrome P450 superfamily and is a monooxygenase with a mass of approximately 57 kDa. CYP3A4 is primarily expressed in the liver and small intestine although it can be found in other tissues at lower levels. This enzyme catalyzes the oxidation of a wide variety of structurally unrelated compounds facilitating their excretion. Proadifen is a known inhibitor of CYP3A4 often used in research to study the effects of enzyme inhibition.
Biological function summary
CYP3A4 is involved in the metabolism of approximately half of all drugs used in clinical settings making it one of the most significant enzymes in drug metabolism. It does not usually function as part of a larger complex but acts individually on various substrates. Besides xenobiotic metabolism CYP3A4 also metabolizes steroids and other endogenous molecules. Climbazole an antifungal agent is a substrate for CYP3A4 demonstrating the enzyme's broad substrate specificity within the body's complex biochemical environment.
Pathways
CYP3A4 plays a central role in the drug metabolism pathway and the synthesis and metabolism of steroids. These pathways involve other cytochrome P450 enzymes including CYP2D6 and CYP2C9 which often work alongside CYP3A4 to metabolize compounds. The pathway's coordinated activities ensure proper drug metabolism and hormone regulation. Understanding the involvement of CYP3A4 in these pathways is critical for drug development and determining drug-drug interactions.
CYP3A4 is associated with conditions such as drug-induced liver injury and variable drug responses. Alterations in CYP3A4 activity can lead to either toxic accumulations or reduced efficacy of medications. Additionally certain liver diseases can alter CYP3A4 expression further complicating treatment outcomes. The enzyme's interaction with other proteins like those of the cytochrome P450 family means that any dysfunctions can impact a wide range of metabolic processes and influence disease progression.