Abcam, ab3649, Anti-Mucin 5AC antibody [45M1]

Size: 20µg / 100µg / 1mg
Anti-Mucin 5AC antibody [45M1] (ab3649) is a mouse monoclonal antibody detecting Mucin 5AC in IHC-P, IHC-Fr, ICC/IF, mIHC . Suitable for Human, Mouse, Rat . - Multiplex IHC validated on the Leica BOND® MAX using Opal reagents - Over 140 publications - Trusted since 2003
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:45M1,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, ICC/IF, mIHC, IHC-FrSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
This product has switched from a hybridoma to recombinant production method on 8
March 2021.
Product Specifications
Anti-Mucin 5AC antibody [45M1] (ab3649) is a mouse recombinant monoclonal antibody and is validated for use in ICC/IF, IHC-Fr, IHC-P, mIHC in human, mouse, rat samples.
Anti-Mucin 5AC antibody [45M1] (ab3649) specifically detects Mucin 5AC (UniProt ID: P98088; Molecular weight: 583kDa) and is sold in a convenient trial size to enable initial testing (20 µg) and larger sizes for subsequent scaling up experiments (100 µg and 1 mg).
Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-Mucin 5AC antibody [45M1] (ab3649) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-Mucin 5AC antibody [45M1] (ab3649) has been cited over 146 times in peer reviewed journals and is trusted by the scientific community.
Anti-Mucin 5AC antibody [45M1] (ab3649) has 6 independent reviews from customers.
Related Products
Conjugation-ready, carrier free format available for antibody clone 45M1 -
ab264494
Antibody clone 45M1 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 647 (
ab309610
ab309611
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Mucin 5AC also known simply as MUC5AC is a high molecular weight glycoprotein with a mass in the megadaltons typically found in the human respiratory gastrointestinal and reproductive tracts. It is a major component of mucus which plays a critical role in protecting and lubricating the epithelial surfaces of these organs. MUC5AC is primarily expressed in the goblet cells of the respiratory epithelium and the stomach lining. Due to its significance researchers often use a MUC5AC ELISA or other detection methods such as tagging with Alexa Fluor 555 to measure its levels in various tissues.
Biological function summary
The functions of MUC5AC are central to the maintenance of the protective mucus barrier on epithelial surfaces preventing pathogen invasion and facilitating the clearance of inhaled particles. It is a part of the gel-forming mucin family and often forms complex structures by polymerizing with other mucins like MUC2 and MUC5B contributing to the viscoelastic properties of mucus. These interactions ensure the formation of a stable and robust mucosal barrier.
Pathways
The regulation of MUC5AC production involves several signaling pathways including the EGFR (epidermal growth factor receptor) pathway and the IL-13/STAT6 pathway. These pathways influence mucin production and secretion particularly in response to growth factors and inflammatory cytokines. Proteins such as 13M1 and 11M1 interact with MUC5AC within these pathways modulating its expression and function. This regulation is essential for normal mucus homeostasis and response to environmental challenges.
MUC5AC has a strong association with respiratory conditions like asthma and chronic obstructive pulmonary disease (COPD). In these diseases the overproduction or altered composition of MUC5AC contributes to airway obstruction and reduced pulmonary function. MUC5AC levels can be indicative of disease severity and are often measured via tests like the 5AC blood test. In these contexts proteins such as 5AC protein and 45M1 are often studied for their roles in facilitating disease progression and as potential therapeutic targets to modulate mucin levels in affected individuals.