Abcam, ab36772, Anti-Spi2A antibody [MoFo 29.2]

Size: 50µg
Hamster Monoclonal Spi2A antibody. Suitable for IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Mouse Serpina3g aa 400 to C-terminus.
Key facts
Host species:Hamster,
Clonality:Monoclonal,
Clone number:MoFo 29.2,
Isotype:IgG2b,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Mouse Serpina3g aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q5I2A0,
Specificity:ab36772 recognises Serine protease inhibitor 2A, or Serpin 2A (spi2A).

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.05% Sodium azideConstituents: PBS, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Spi2A also known as Serpinb9 is a serine protease inhibitor that plays a mechanical role in regulating protease activity. Its molecular weight is approximately 42 kDa. Scientists find Spi2A expression in the cytoplasm of various immune cells including T lymphocytes and natural killer (NK) cells. It is expressed in specific tissues such as the spleen and lymph nodes which indicates its role in immune function.
Biological function summary
Spi2A acts by inhibiting the activity of granzyme B a serine protease released by cytotoxic T cells and NK cells. Through its inhibition of granzyme B Spi2A protects target cells from apoptosis induced by the immune system. It is not part of a larger protein complex but functions as a standalone inhibitor. This activity allows immune cells to regulate cell death maintaining homeostasis and preventing excessive tissue damage during immune responses.
Pathways
Spi2A contributes significantly within immune regulatory pathways by influencing the apoptosis pathway. It primarily affects pathways associated with the immune response specifically in the management of cell-mediated cytotoxicity. Spi2A also interacts with other proteins involved in immune regulation such as caspases which play critical roles in apoptosis.
The dysregulation of Spi2A has been linked to autoimmune diseases and certain cancers. Aberrant Spi2A function can contribute to autoimmune conditions by altering normal immune cell apoptosis leading to inappropriate cell survival. Additionally Spi2A interactions with proteins like granzyme B can affect the progression of certain cancers as cancer cells may express higher levels of Spi2A to evade immune-mediated apoptosis. Understanding Spi2A's regulation is therefore essential in these pathological conditions.