Abcam, ab36844, Anti-VEGF Receptor 1 + VEGF Receptor 2 antibody

Size: 100µL
Rabbit Polyclonal VEGF Receptor 1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 13 publications. Immunogen corresponding to Synthetic Peptide within Mouse Flt1 aa 800-900.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Mouse,
Applications:ICC/IF, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Mouse Flt1 aa 800-900. The exact immunogen used to generate this antibody is proprietary information.P35969,
Specificity:This antibody is specific for VEGFR 1 with significant cross-reactivity with the VEGFR 2 protein.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Purification notes-ab36844 is an affinity purified IgG., Storage buffer-pH: 7.4Preservative: 0.05% Sodium azide, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The VEGF Receptor 1 (known as Flt-1) and VEGF Receptor 2 (known as KDR/Flk-1) are important receptors that mediate angiogenesis. Flt-1 and KDR have masses of approximately 180 kDa and 210 kDa respectively. They express mainly on endothelial cells and are also present in some hematopoietic cells pericytes and specific tumor cells. Both receptors bind vascular endothelial growth factor (VEGF) a signal protein produced by cells that stimulates the formation of blood vessels.
Biological function summary
Flt-1 and KDR are part of receptor tyrosine kinase families and form complexes essential to regulating endothelial cell function. When VEGF binds to these receptors it leads to their dimerization and activation of their intrinsic kinase domain. This activation triggers multiple downstream signaling cascades that govern cell proliferation migration and survival. VEGF binding to KDR especially plays a significant role in vascular permeability and endothelial cell growth.
Pathways
VEGF Receptor 1 and 2 engage primarily in the VEGF signaling pathway impacting angiogenesis and vasculogenesis. The signal transduction cascade involves multiple proteins including PI3K and PLCγ leading to different cellular responses. The receptors' activity also interlinks with the MAPK/ERK pathway connecting with other proteins such as Raf-1 important for cell division and differentiation.
Aberrant signaling through VEGF Receptors 1 and 2 associates with cancer and age-related macular degeneration (AMD). Overexpression or excessive activation of these receptors contributes to tumor angiogenesis supporting cancerous cell growth and metastasis in various cancers. In AMD abnormal angiogenesis in the retina leads to vision loss where both Flt-1 and KDR play roles in pathological neovascularization. Pharmacological inhibition of these receptors such as with anti-VEGF therapies shows effectiveness in treating these conditions.