Abcam, ab37152, Anti-XBP1 antibody

Size: 100µg
Anti-XBP1 antibody (ab37152) is a rabbit polyclonal antibody detecting XBP1 in Western Blot, IHC-P, IHC-Fr, ICC/IF . Suitable for Human, Mouse, Rat . - KO validated for confirmed specificity - Over 160 publications - Trusted since 2006
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human, Rat,
Applications:ICC, ICC/IF, IHC-P, IHC (PFA fixed), IHC-Fr, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human XBP1. The exact immunogen used to generate this antibody is proprietary information.P17861,
Specificity:XBP-1 antibody will recognize both isoforms (non-spliced and spliced) of XBP-1.

Product details:
Product Specifications
Anti-XBP1 antibody (ab37152) is a rabbit polyclonal antibody and is validated for use in ICC, ICC/IF, IHC (PFA fixed), IHC-Fr, IHC-P, WB in human, mouse, rat samples.
Anti-XBP1 antibody (ab37152) specifically detects XBP1 (UniProt ID: P17861; Molecular weight: 29kDa) and is sold in 100 µg selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-XBP1 antibody (ab37152) has high sensitivity and specificity.
Anti-XBP1 antibody (ab37152) has been cited over 165 times in peer reviewed journals and is trusted by the scientific community.
Anti-XBP1 antibody (ab37152) has 28 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
XBP1 also known as X-box binding protein 1 is a transcription factor with a mass of approximately 33 kDa. It is mainly expressed in the endoplasmic reticulum of cells where it plays a significant role in regulating the unfolded protein response (UPR). XBP1 exists in two forms: an unspliced form (XBP1u) and a spliced form (XBP1s). The splicing of XBP1 mRNA is mediated by the endoribonuclease IRE1 and is a response to endoplasmic reticulum stress. This process results in a shift of the reading frame which leads to the production of a new functional transcription factor.
Biological function summary
The action of XBP1 participates in processes critical for cell survival under stress conditions particularly in the UPR. It activates genes involved in protein folding secretion and degradation to alleviate stress within the endoplasmic reticulum. XBP1 acts as part of the larger mechanism that ensures cellular homeostasis by maintaining protein quality control. It forms a complex network with other transcription factors ensuring an integrated response to stress stimuli in various tissues including pancreatic beta cells and immune cells.
Pathways
XBP1 functions intricately within the UPR and is linked to the insulin signaling pathway. It works alongside proteins such as ATF6 and PERK to coordinate the cellular response to endoplasmic reticulum stress and sustain essential cellular processes. The IRE1-XBP1 pathway represents one arm of the UPR and shows interaction with C/EBP homologous protein (CHOP) as part of the stress signaling pathways modulating apoptosis if adaptation fails. XBP1 also influences lipid metabolism and cellular differentiation through its pathway connections impacting wide-ranging physiological processes.
Dysfunctions of XBP1 have associations with metabolic diseases and multiple myeloma. Altered XBP1 activity links to diabetes due to its role in protein homeostasis in pancreatic beta cells involving modulation of insulin secretion and sensitivity. In cancer particularly multiple myeloma XBP1 interacts with proteins such as B-cell maturation antigen (BCMA) influencing cell survival and proliferation. Targeting the XBP1 signaling axis shows potential as a therapeutic strategy for managing disorders resulting from dysregulated protein folding and stress responses.