Abcam, ab37274, Anti-Cholecystokinin 8 antibody [27F3..0,5D10]

Size: 100µL
Mouse Monoclonal Cholecystokinin antibody. Suitable for ELISA, IHC-FoFr and reacts with Rat samples. Cited in 16 publications. Immunogen corresponding to Synthetic Peptide within Human Cholecystokinin.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:27F3..0,5D10,
Isotype:IgG2a,
Light chain type:kappa,
Carrier free:No,
Reacts with:Rat,
Applications:ELISA, IHC-FoFrSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human Cholecystokinin. The exact immunogen used to generate this antibody is proprietary information.P06307,
Specificity:Reacts with CCK-8 and the other CCK analogs with identical C-terminal ends as well as Gastrin 17. No reactivity was observed with Human Heparin cofactor II.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Constituents: PBS, 2.9% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Cholecystokinin-8 (CCK-8) a well-known peptide hormone influences various physiological processes. At a molecular level CCK-8 has a mass of approximately 1150 Da and acts primarily by binding to specific receptors such as the CCK-A and CCK-B receptors. These receptors distribute broadly in tissues like the gastrointestinal tract and the brain. Its alternative name is sulfated octapeptide of Cholecystokinin but researchers often refer to it as CCK-8. The peptide's expression in these areas enables it to mediate digestive enzyme secretion satiety and neurotransmission.
Biological function summary
This active fragment of the larger cholecystokinin peptide exhibits functions beyond digestion. Cholecystokinin-8 plays a role in stimulating digestion by influencing the release of digestive enzymes from the pancreas and bile from the gallbladder. It interacts with other neuropeptides within the central nervous system affecting anxiety and feeding behavior. It does not act as part of a larger complex but participates in straightforward receptor-ligand interactions allowing it to exert its effects swiftly.
Pathways
Cholecystokinin-8 interacts significantly in the digestion-related and neurological pathways. In the gastrointestinal system CCK-8 is involved in the digestion pathway by promoting efficient nutrient absorption. Additionally it participates in the modulation of appetite in the satiety pathway linked closely with leptin and its regulatory effects on hunger. These pathways add to the multifunctionality of CCK-8 making it essential for maintaining homeostasis within the body's metabolic processes.
Cholecystokinin-8 has known associations with obesity and panic disorder. In obesity CCK-8's role in regulating satiety can be disrupted leading to overeating and weight gain. The peptide interacts with leptin in this context affecting energy balance and appetite control. In panic disorder alterations in CCK-8 levels can affect the central nervous system potentially triggering anxiety-related phenotypes similar to its interaction with serotonin receptors. These associations highlight CCK-8's potential as a target for therapeutic interventions in these conditions.