Abcam, ab3882, Anti-BAF53A antibody - ChIP Grade

Size: 100µg
Rabbit Polyclonal ACL6A antibody. Suitable for ChIP, WB and reacts with Human, Mouse, Rat samples. Cited in 13 publications.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:ChIP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:In Western blot, ab3882 detects BAF53 and Tip60-TAP in total extracts of Tip60-TAP.  Following TEV elution of Tip60-TAP ab3882 only detects BAF53. From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. From July 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein BAF53A also known as ACTL6A plays an important role in gene expression through chromatin remodeling. It is a subunit of the SWI/SNF-like BAF complex that is responsible for altering chromatin structure making it easier for transcription factors to access DNA. BAF53A has a molecular mass of approximately 53 kDa. It is highly expressed in neurons and plays an essential role in neurodevelopment and the regulation of neuronal gene expression.
Biological function summary
BAF53A is an integral component of the BAF chromatin remodeling complex. This complex modifies the positioning and accessibility of nucleosomes on chromatin thereby influencing gene transcription processes. BAF53A contributes to the regulation of genes involved in neuronal differentiation and development. The protein plays a significant role in neural stem cell proliferation and the regulation of progenitor cell fate.
Pathways
The BAF53A protein participates actively in the neural development and chromatin remodeling pathways. It interacts closely with the BAF complex to regulate the transcription of genes essential for neural differentiation and development. The BAF complex which includes BAF53A associates with proteins such as BRG1 and SMARCC1 facilitating the unfolding of chromatin to affect gene expression positively.
BAF53A is associated with neurodevelopmental disorders and certain types of cancer. Altered expression or mutations in BAF53A have been linked to neurodevelopmental issues contributing to conditions such as Autism Spectrum Disorders. Additionally overexpression of BAF53A connects to cancer particularly in glioblastoma where it influences tumor progression. In these contexts BAF53A interacts with oncogenes and tumor suppressor proteins playing a role in disease pathogenesis.