Abcam, ab4074, Anti-alpha Tubulin antibody - Loading Control

Size: 100µg / 250µg
Anti-alpha Tubulin antibody - Loading Control (ab4074) is a rabbit polyclonal antibody detecting alpha Tubulin in Western Blot, ICC/IF . Suitable for Chicken, Chinese hamster, Cow, Human, Mouse, Rat . - Over 430 publications - Trusted since 2005
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Chicken, Cow, Human, Chinese hamster,
Applications:ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody detects a single band in cell lines, corresponding to alpha tubulin.

Product details:
Anti-alpha Tubulin antibody - Loading Control (ab4074) is a rabbit polyclonal antibody and is validated for use in ICC/IF, WB in drosophila melanogaster, human, mouse, pig, rat samples.
Anti-alpha Tubulin antibody - Loading Control (ab4074) has been cited over 431 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-alpha Tubulin antibody - Loading Control (ab4074) has high sensitivity and specificity.
Anti-alpha Tubulin antibody - Loading Control (ab4074) has 28 independent reviews from customers.
alpha Tubulin antibodies are often used as loading controls in Western Blot. Anti-alpha Tubulin antibody - Loading Control has been verified in Western Blot samples and detects a band at 50kDa Molecular weight.
Anti-alpha Tubulin antibody (Anti-TUBA4A antibody) (ab4074) specifically detects alpha Tubulin (UniProt ID: P68366; Molecular weight: 50kDa) and is sold in 100 µg and 250 µg selling sizes.
Alpha Tubulin (TUBA4A) is a fundamental component of microtubules, essential for maintaining the cytoskeleton and facilitating cell division. The alpha tubulin molecular weight is approximately 50 kDa, making it a critical marker in various studies. Abnormalities in alpha Tubulin are linked to neurodegenerative diseases and cancer, highlighting its disease relevance. Our A-tubulin antibody is a valuable tool for research, aiding in the detection and study of alpha Tubulin pathways. Understanding the role of alpha Tubulin, including its post-translational modifications, is crucial for developing targeted therapies.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Alpha tubulin also known as DM1A is an important component of the structural unit called microtubules. This protein weighs approximately 50 kDa and acts as a building block in the cytoskeleton. Alpha tubulin is expressed in various cell types across many organisms playing a fundamental role in maintaining cell shape and enabling intracellular transport. Researchers often acetylate alpha tubulin to study post-translational modifications and its effects on microtubule dynamics.
Biological function summary
Alpha tubulin combines with beta tubulin to form the tubulin dimer which is the basic unit of microtubule polymerization. These dimers assemble into microtubule filaments integral in numerous cellular processes. As part of the cytoskeleton network alpha tubulin facilitates roles in mitosis and meiosis providing spindle structures necessary for chromosome separation. Its participation in cell signaling pathways is noteworthy as it interacts with motor proteins like kinesins and dyneins to aid cellular activities.
Pathways
Alpha tubulin holds critical roles in microtubule-associated processes within the cytoskeleton and intracellular transport pathways. It interacts with the MAP (microtubule-associated protein) family which modulates microtubule stability and impacts cell division and trafficking. Moreover the microtubule pathway involves proteins like tau which bind to stabilize microtubules and are implicated in cellular dynamics and signaling.
Alpha tubulin is relevant in neurodegenerative diseases such as Alzheimer's disease and cancer. Aberrant modifications or mutations in alpha tubulin or associated proteins like tau can disrupt microtubule stability contributing to pathological conditions. In cancer altered expression of alpha tubulin affects cell division leading to tumor progression and chemoresistance. Researchers study alpha tubulin using techniques such as immunofluorescence with Alexa fluor 555 to understand its role in disease better.