Abcam, ab40742, HRP Anti-alpha Tubulin antibody [DM1A] - Loading Control

Size: 100µL
Anti-alpha Tubulin antibody [DM1A] - Loading Control - HRP conjugated (ab40742) is a mouse monoclonal antibody detecting alpha Tubulin in Western Blot, IHC-P . Suitable for Human, Mouse, Rat, Xenopus laevis . - Over 70 publications - Trusted since 2007
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:DM1A,
Isotype:IgG,
Conjugation:HRP,
Carrier free:No,
Reacts with:Mouse, Rat, Human, Xenopus laevis,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
HRP Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab40742) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Mouse, Rat, Xenopus laevis samples.
What is the molecular weight of alpha Tubulin?
Anti-alpha Tubulin [DM1A] - Loading Control HRP (ab40742) is often used as a loading control in Western blot. It specifically detects a band for alpha Tubulin (UniProt: P02552) at a molecular weight of 50kDa.
Trusted by the scientific community
Anti-alpha Tubulin [DM1A] - Loading Control HRP (ab40742) was first used in a scientific publication in 2007 and has been cited over 70 times in peer-reviewed journals.
Related products
Antibody clone DM1A is also available pre-conjugated to a variety of labels for your convenience – Anti-alpha Tubulin HRP [DM1A] - Loading Control (ab40742).
Other related products
We have a range of other formats of antibody clone [DM1A] also available for your convenience:
ab7291
, HRP - ab40742, Alexa Fluor® 488 -
ab195887
, Alexa Fluor® 594 -
ab195889
, Carrier free -
ab264493

Properties and Storage Information:
Form-Liquid, Purity-IgG fraction, Storage buffer-Preservative: 0.1% Proclin 300 SolutionConstituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Alpha tubulin also known as DM1A is an important component of the structural unit called microtubules. This protein weighs approximately 50 kDa and acts as a building block in the cytoskeleton. Alpha tubulin is expressed in various cell types across many organisms playing a fundamental role in maintaining cell shape and enabling intracellular transport. Researchers often acetylate alpha tubulin to study post-translational modifications and its effects on microtubule dynamics.
Biological function summary
Alpha tubulin combines with beta tubulin to form the tubulin dimer which is the basic unit of microtubule polymerization. These dimers assemble into microtubule filaments integral in numerous cellular processes. As part of the cytoskeleton network alpha tubulin facilitates roles in mitosis and meiosis providing spindle structures necessary for chromosome separation. Its participation in cell signaling pathways is noteworthy as it interacts with motor proteins like kinesins and dyneins to aid cellular activities.
Pathways
Alpha tubulin holds critical roles in microtubule-associated processes within the cytoskeleton and intracellular transport pathways. It interacts with the MAP (microtubule-associated protein) family which modulates microtubule stability and impacts cell division and trafficking. Moreover the microtubule pathway involves proteins like tau which bind to stabilize microtubules and are implicated in cellular dynamics and signaling.
Alpha tubulin is relevant in neurodegenerative diseases such as Alzheimer's disease and cancer. Aberrant modifications or mutations in alpha tubulin or associated proteins like tau can disrupt microtubule stability contributing to pathological conditions. In cancer altered expression of alpha tubulin affects cell division leading to tumor progression and chemoresistance. Researchers study alpha tubulin using techniques such as immunofluorescence with Alexa fluor 555 to understand its role in disease better.