Abcam, ab409, Anti-N-epsilon acetyl Lysine antibody [11A1]

Size: 100µg
Mouse Monoclonal N-epsilon acetyl Lysine antibody. Suitable for ELISA, WB, IHC-P and reacts with Modified Amino Acid samples. Cited in 9 publications.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:11A1,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:No,
Applications:ELISA, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Product details:
The ability of this antibody to fix complement is unknown.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Constituents: 2% Sucrose, 1.21% Tris, 0.75% Glycine, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
N-epsilon acetyl Lysine often known as acetylated lysine is a modified amino acid resulting from the acetylation of the lysine residue. This modification typically occurs as part of protein post-translational modifications which influence protein function and interaction. N-epsilon acetyl Lysine itself is not a standalone protein with a specific mass; rather it fits into larger proteins as an acetylation site. These acetylation events frequently take place in the nucleus primarily affecting histone proteins within chromatin structures impacting gene expression regulation.
Biological function summary
Acetylation of lysine residues plays an important role in regulating gene expression and chromatin dynamics. Acetylation neutralizes the positive charge of lysine affecting histone's binding affinity for DNA and other regulatory proteins. This modification typically leads to chromatin relaxation facilitating access to transcription factors and promoting gene transcription. N-epsilon acetyl Lysine often appears within the context of larger multiprotein complexes like the histone acetyltransferase complex which includes other proteins such as CBP/p300.
Pathways
N-epsilon acetyl Lysine is critical in chromatin remodeling and transcriptional regulation pathways. Acetylation interacts significantly with the transcriptional coactivators p300/CBP which transfer acetyl groups to specific lysines on histone tails directly impacting gene transcription. Additionally it is involved in the regulation of the cell cycle through pathways that include the p21 protein which influences cellular proliferation and differentiation.
Acetylation of lysine residues is implicated in cancer and neurological disorders. In cancer altered acetylation patterns result in aberrant gene expression as seen in proteins interacting with p53 an important tumor suppressor gene. Similarly in neurological disorders like Alzheimer's disease dysregulated lysine acetylation affects the proteins tau and amyloid precursor protein (APP) contributing to disease pathology. Understanding the role of N-epsilon acetyl Lysine within these contexts aids in pioneering targeted therapeutic strategies.