Abcam, ab413, Anti-dimethyl Arginine antibody [21C7]

Size: 100µg
Mouse Monoclonal dimethyl Arginine antibody. Suitable for IP, ChIP, WB and reacts with Modified Amino Acid samples. Cited in 12 publications.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:21C7,
Isotype:IgM,
Light chain type:kappa,
Carrier free:No,
Applications:IP, ChIP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:This antibody reacts with free and bound asymmetric NG-NG-dimethyl arginine. It is not known whether the antibody reacts with symmetric dimethyl arginine.

Product details:
This antibody will be of use in chromatin and transcription factor research to monitor methylation status. Since it is specific to dimethyl arginine it can be used in parallel with
(detects monomethyl and dimethyl arginine) and
(detects monomethyl arginine only) to monitor the exact modification status.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Constituents: 2% Sucrose, 1.21% Tris, 0.75% Glycine, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Dimethyl Arginine often referred to as asymmetric dimethylarginine (ADMA) is an endogenously produced molecule in the body. The molecular mass of dimethyl arginine usually depends on its specific dimethylated form with the molecule having different variants such as symmetric dimethylarginine (SDMA) and monomethylarginine (MMA). While dimethyl arginine is expressed in various tissues it predominantly arises as a byproduct of protein methylation processes. Protein arginine methyltransferases (PRMTs) enzymes responsible for these modifications mediate its formation influencing numerous physiological functions.
Biological function summary
Dimethyl arginine modulates nitric oxide (NO) signaling by inhibiting nitric oxide synthase (NOS) enzymes which are vital for the production of NO—an important player in vasodilation and immune response. NO production requires L-arginine as a substrate and competition from dimethylated arginines reduces NO availability. This molecule though not a part of any large complex significantly impacts endothelial function and cell proliferation by affecting NO synthesis and cellular response to growth factors.
Pathways
Dimethyl arginine plays an integral role in the regulation of the cardiovascular system and is involved in the urea cycle and polyamine pathways which are important for removing excess nitrogen and supporting cell growth respectively. The enzymes related to these pathways such as endothelial nitric oxide synthase (eNOS) and dimethylarginine dimethylaminohydrolase (DDAH) illustrate the interconnectedness of dimethyl arginine in maintaining vascular health and cellular homeostasis. The balance between dimethyl arginine and arginine levels affect the efficiency of the pathways indicating their influence on systemic health.
Dimethyl arginine links to cardiovascular diseases and chronic kidney disease. Its elevated levels often point to endothelial dysfunction contributing to the pathogenesis of atherosclerosis due to impaired NO-mediated vasodilation. Additionally kidney dysfunction can result in decreased dimethylarginine clearance exacerbating cardiovascular issues. Proteins such as eNOS and DDAH play pivotal roles in these conditions as they modulate the effects of dimethyl arginine on vascular and kidney health highlighting the importance of managing its levels in disease prevention and treatment.