Abcam, ab41552, Anti-Myocilin antibody

Size: 50µg
Rabbit Polyclonal Myocilin antibody. Suitable for IP, WB, IHC-P and reacts with Mouse, Rat, Monkey, Human samples. Cited in 20 publications. Immunogen corresponding to Synthetic Peptide within Human MYOC aa 1-50 conjugated to Keyhole Limpet Haemocyanin.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Monkey, Human, Rat,
Applications:IP, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human MYOC aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.Q99972,
Specificity:The antibody does not cross react with any other protein in RPE cells.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Myocilin also known as Trabecular Meshwork Inducible Glucocorticoid Response protein (TIGR) is a protein mainly involved in ocular processes. It weighs about 55 kDa and contains several key domains including a leucine zipper motif and an olfactomedin-like domain. Myocilin is abundantly expressed in the trabecular meshwork of the eye skeletal muscle heart and brain. The protein appears in both intracellular and secreted forms and undergoes glycosylation.
Biological function summary
This protein plays an important role in the regulation of intraocular pressure by influencing the outflow of aqueous humor through the trabecular meshwork. It is also known to be part of a protein complex that interacts with other extracellular matrix components to maintain the structural integrity of tissues. Beyond the eye Myocilin similarly contributes to cellular processes in other tissues assisting in maintaining cellular structure and integrity.
Pathways
Myocilin functions within the Wnt signaling pathway and extracellular matrix-receptor interaction pathway. These pathways are significant in regulating cell growth adhesion and differentiation in various tissues. In the Wnt signaling pathway Myocilin interacts with proteins such as Frizzled receptors affecting the downstream effects on cell behavior and fate. In the extracellular matrix pathway Myocilin associates with collagens and laminins influencing tissue architecture and dynamics.
Myocilin plays a significant role in glaucoma and Myocilin-related ocular hypertension. Glaucoma often involves elevated intraocular pressure due to impaired aqueous humor outflow in which mutated Myocilin may not function properly affecting cellular interactions and contributing to disease progression. Links have been identified with proteins like optineurin in the context of glaucoma where these proteins can collectively influence disease pathophysiology and lead to optic nerve damage if dysregulated.