Abcam, ab41969, Anti-EHMT1/GLP antibody [B0422]

Size: 100µL
Mouse Monoclonal EHMT1/GLP antibody. Suitable for ICC/IF and reacts with Mouse samples. Cited in 32 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Ehmt1 aa 100-250.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:B0422,
Isotype:IgG2a,
Carrier free:No,
Reacts with:Mouse,
Applications:ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Mouse Ehmt1 aa 100-250. The exact immunogen used to generate this antibody is proprietary information.Q5DW34

Product details:
This product was changed from ascites to tissue culture supernatant on 3
April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties and Storage Information:
Form-Liquid, Purity-Tissue culture supernatant, Storage buffer-pH: 7Preservative: 0.1% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
EHMT1 also known as GLP (G9a-like protein) is a histone methyltransferase involved in the methylation of histone H3 on lysine 9 a modification leading to transcriptional repression. The EHMT1 protein has a mass of approximately 150 kDa and is expressed in numerous tissues including the brain and heart. It functions as part of a larger complex and acts in the modification of chromatin structure and gene expression regulation.
Biological function summary
EHMT1/GLP plays a critical role in cellular processes such as development and differentiation. It forms a complex with G9a another histone methyltransferase to facilitate these functions. This complex cooperates in controlling gene silencing and the preservation of cellular identity. Such complex mechanisms lead to alterations in gene expression patterns that are essential for normal cellular operation.
Pathways
The methylation activity of EHMT1/GLP participates in the epigenetic regulation pathway and chromatin remodeling pathways. Within these pathways it interacts closely with proteins like HP1 which binds to methylated histones to promote gene silencing. The connections with pathways that influence gene expression affirm its role in maintaining cellular processes like proliferation and embryonic development.
Researchers connect EHMT1/GLP to Kleefstra syndrome—a condition characterized by intellectual disability and developmental delays. It is also associated with some cancer types due to its epigenetic regulation influences. In these contexts abnormal EHMT1/GLP activity often involving other proteins like G9a can contribute to the disruptions in gene regulation that lead to disease manifestations.