Abcam, ab42364, Anti-OPA1 antibody

Size: 100µg
Rabbit Polyclonal OPA1 antibody. Suitable for IP, WB and reacts with Rat, Mouse, Human samples. Cited in 179 publications.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
OPA1 also known as optic atrophy 1 is a dynamin-related GTPase protein important for mitochondrial fusion. OPA1 has a molecular weight of about 120 kDa and is present mostly in the inner mitochondrial membrane. It promotes the maintenance of mitochondrial DNA cristae structures and the modulation of mitochondrial dynamics. Expression of OPA1 occurs in tissues with high energy demands including the retina brain and muscles. Detection of the OPA1 protein can be done using techniques such as Western blot and it reveals different isoforms generated through alternative splicing.
Biological function summary
Mitochondrial dynamics involving OPA1 ensure energy production efficiency and cell health. OPA1 plays a role in mitochondrial fusion by forming a complex with mitofusins MFN1 and MFN2. This complex maintains the integrity of mitochondrial networks facilitates proper respiratory function and prevents apoptosis by regulating cristae junctions. It also participates in the stress response particularly in the preservation of the mitochondrial structure and function under challenging conditions.
Pathways
OPA1 integrates into the mitochondrial fusion and fission pathways important for cellular energy metabolism. It works alongside proteins like DRP1 in balancing these processes. The involvement in these pathways is essential for cellular adaptation to metabolic needs and stress. OPA1 also has a relationship with the PINK1/Parkin pathway where its regulation affects mitophagy a process of clearing damaged mitochondria. These interactions highlight the importance of OPA1 in maintaining cellular and mitochondrial homeostasis.
Mutations in OPA1 have been linked to autosomal dominant optic atrophy and a range of neurodegenerative conditions. The protein’s dysfunction leads to the degeneration of the retinal ganglion cells and their axons resulting in vision loss. OPA1 also shows connections to disorders like Charcot-Marie-Tooth disease where its interaction with other proteins like MFN2 plays a role. Deficiency or dysfunction of OPA1 disrupts mitochondrial dynamics leading to cellular energy deficits and contributing to disease pathophysiology.