Abcam, ab42497, Anti-IFT88 antibody

Size: 100µg
Goat Polyclonal IFT88 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Human, Rat samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human IFT88 aa 800 to C-terminus.
Key facts
Host species:Goat,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Rat, Human,
Applications:WB, ICC/IF, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human IFT88 aa 800 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q13099,
Specificity:This antibody is expected to recognise both reported isoforms (NP_783195.2; NP_006522.2).

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide., Storage buffer-pH: 7.3Preservative: 0.02% Sodium azideConstituents: 0.5% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
IFT88 also known as Intraflagellar transport protein 88 is an essential component of the intraflagellar transport (IFT) mechanism. This protein has a mass of approximately 88 kDa and is expressed in ciliated cells across various tissues. Cilia are critical structures in cellular function and IFT88 operates as part of the IFT-B complex playing a role in the assembly and maintenance of cilia. Its localization primarily occurs in the cilia where it functions as a transporter of protein cargos necessary for cilia construction and function.
Biological function summary
The role of IFT88 within the context of cilia involves the facilitation of bidirectional movement along the axoneme which is the central shaft of a cilium. As part of the IFT-B complex IFT88 aids in the transport of tubulin and other ciliary components from the cell body to the ciliary tip ensuring cilia's structural integrity and functional viability. Additionally IFT88 supports signal transduction processes associated with ciliary functions including sensing extracellular signals and maintaining cellular signaling pathways.
Pathways
IFT88 has significant involvement in the Hedgehog and Wnt signaling pathways. These pathways are integral for cell differentiation proliferation and embryonic development. IFT88 collaborates with proteins such as Gli transcription factors in Hedgehog signaling and interacts with various cytosolic components in Wnt signaling showing its versatile role in cellular processes. Through these interactions IFT88 indirectly influences the outcome of these signaling pathways impacting tissue patterning and cell fate decisions during development.
IFT88 mutations are linked to ciliopathies like polycystic kidney disease and Bardet-Biedl syndrome. These conditions demonstrate defects in ciliary function where IFT88 dysfunction results in abnormal cellular processes. The connection between IFT88 and these diseases extends to its interactions with proteins such as polycystin-1 and polycystin-2 in kidney development and function. Such associations emphasize the critical role of IFT88 in maintaining normal cellular and organ function whereby its impairment leads to profound developmental and physiological anomalies.