Abcam, ab42631, Recombinant HDAC3+NCOR2 complex protein

Size: 50µg
Recombinant HDAC3+NCOR2 complex protein is a Human protein, expressed in Baculovirus, with >90%, suitable for Inhib.
Key facts
Purity:>90% SDS-PAGE,
Expression system:Baculovirus,
Tags:Tag free,
Applications:InhibSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Activity: 110 U/ug. One U =1 pmol/min, Assay condition: 25 mM Tris/Cl, pH8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 30 uM Biomol substrate (Catalog number KI177), and 1 ng/ul HDAC3/NcoR2. Incubation condition: 30 min at 37°C.,
Accession:O15379,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 10% Glycerol (glycerin, glycerine), 0.8004% Sodium chloride, 0.79% Tris HCl, 0.614% Glutathione

Product details:
Co-expressed in a Baculovirus infected Sf9 cell expression system.

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The HDAC3+NCOR2 complex refers to the association of histone deacetylase 3 (HDAC3) and nuclear receptor corepressor 2 (NCOR2) also known as SMRT. HDAC3 is an enzyme with a mass of about 48 kDa. It mainly resides in the nucleus where it removes acetyl groups from lysine residues on histone proteins. This action regulates chromatin structure and gene expression. NCOR2 collaborates with HDAC3 acting as a scaffold protein that helps recruit other corepressors and transcription factors to the complex. Both proteins are widely expressed in various tissues having key functions in processes like transcriptional repression.
Biological function summary
The HDAC3+NCOR2 complex plays an essential role in regulating gene expression. As part of a complex it modifies chromatin properties influencing transcriptional repression and activation. The deacetylation activity of HDAC3 controls the accessibility of genetic material for transcription. Through association with NCOR2 it recruits additional corepressors like TBL1 and TBLR1 forming a multi-protein network for efficient chromatin remodeling. This dynamic regulation is critical for cellular processes such as differentiation proliferation and metabolism.
Pathways
The HDAC3+NCOR2 complex operates within significant signaling pathways such as the Notch and NF-κB pathways. In the Notch signaling pathway it interacts with proteins like CSL/RBP-Jk governing the transition between active and repressive states of target genes involved in developmental processes. In the NF-κB pathway the complex affects the transcription of genes involved in immune response and inflammation. NCOR2 through its scaffold function links HDAC3 to additional transcriptional regulators reinforcing the complex's impact on diverse cellular signaling networks.
The HDAC3+NCOR2 complex shows significant connections to cancer and neurodegenerative diseases. The complex's dysregulation can lead to aberrant expression of genes that control cell cycle and apoptosis contributing to oncogenesis in cancers like leukemia and breast cancer. Additionally its involvement in Alzheimer's disease has been noted with disrupted activity affecting neuronal survival and function. In cancer related proteins such as HDAC1 and HDAC2 may also participate in compensatory mechanisms or pathways due to the interplay with HDAC3+NCOR2 highlighting its potential as a therapeutic target.