Abcam, ab4463, Anti-SA2 antibody

Size: 50µg
Goat Polyclonal SA2 antibody. Suitable for ICC, IP, WB and reacts with Human, Mouse samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human STAG2 aa 1100-1200.
Key facts
Host species:Goat,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IP, WB, ICCSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human STAG2 aa 1100-1200. The exact immunogen used to generate this antibody is proprietary information.Q8N3U4,
Specificity:We have data to indicate that this antibody may not cross react with Xenopus laevis. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7 - 8Preservative: 0.1% Sodium azideConstituents: PBS, 1.815% Tris, 1.764% Sodium citrate, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The SA2 protein also known as stromal antigen 2 is a component of the cohesin complex playing a significant role in chromosomal dynamics. With an approximate molecular weight of 152 kDa SA2 exhibits high expression in the nucleus of proliferating cells particularly during interphase. This cohesin component contributes critically to maintaining sister chromatid cohesion and ensuring proper segregation during cell division serving as a functional partner in the cohesin complex with other components like SMC1 SMC3 and RAD21.
Biological function summary
SA2 plays an important role in regulating gene expression by forming loops that connect different genomic regions. SA2 as a member of the cohesin complex also participates in DNA repair by facilitating accurate recombination enhancing genome stability. This involvement is essential for maintaining chromatin structure which influences diverse cellular processes. Its expression can be an important modulator in the dynamic environment of cellular proliferation and differentiation.
Pathways
SA2 functions integrally within the cohesin-mediated sister chromatid cohesion pathway and the DNA damage response pathway. In these pathways SA2 teams with proteins such as CTCF in insulator function regulation and BRCA1 in DNA repair mechanisms. The cohesin-SA2 interaction with these proteins ensures proper chromosomal architecture and integrity which are essential for the accurate transmission of genetic material across generations of cells.
SA2 mutations or dysregulations have been linked to Cornelia de Lange Syndrome (CdLS) and certain cancers. In CdLS SA2 mutations disrupt normal cohesin function affecting developmental processes. In cancer contexts abnormal SA2 function or expression can lead to improper DNA repair contributing to genomic instability and tumorigenesis. It is often studied in concert with other cohesin proteins and regulatory elements like NIPBL which provides insights into cohesin-related pathologies and potential therapeutic targets.