Abcam, ab45381, Anti-p38 (phospho T180 + Y182) antibody [M139]

Size: 100µL
Mouse Monoclonal MK14 phospho Y182 + T180 antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 40 publications. Immunogen corresponding to Synthetic Peptide within Rat Mapk14 phospho Y182 + T180 conjugated to Keyhole Limpet Haemocyanin.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:M139,
Isotype:IgG1,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Rat Mapk14 phospho Y182 + T180 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.P70618,
Specificity:This antibody detects a 42 kDa protein corresponding to the apparent molecular mass of p38α. This peptide sequence is highly conserved in the p38β,g, and d MAPKs, and is identical in human and mouse p38α.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Purification notes-Mouse monoclonal purified with protein A chromatography., Storage buffer-Preservative: 0.05% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
P38 also known as MAPK14 is a member of the mitogen-activated protein kinase (MAPK) family. It functions by phosphorylating various downstream substrates and plays a role in cellular responses. The molecular weight of p38 is approximately 38 kDa. This protein is widely expressed across many tissues including heart brain and lungs. Its activation by stimuli such as cytokines and stress factors helps regulate inflammation and cell cycle control.
Biological function summary
P38 is involved in several cellular processes such as inflammation cell differentiation and apoptosis. It often functions as part of a MAPK signaling complex where it serves a critical role in transmitting signals from the cell surface to the nucleus. It interacts with upstream kinases for activation and affects cellular responses by phosphorylating transcription factors and other protein kinases. Through experiments using techniques like p38 western blot and alpha ELISA scientists can monitor its activity and understand its role in cellular physiology.
Pathways
P38 signaling is integral to both the MAPK and NF-kB pathways. It helps mediate several cellular responses including inflammation and stress responses. Within these pathways p38 interacts with other proteins such as JNK and ERK which helps regulate adaptive and innate immune responses. These interactions ensure distinct yet overlapping signaling responses necessary for cellular homeostasis.
P38 plays a role in conditions such as rheumatoid arthritis and cancer. In rheumatoid arthritis p38 contributes to inflammatory processes promoting the production of pro-inflammatory cytokines. In cancer its role varies; while sometimes promoting cancer cell apoptosis it may also aid in tumor survival and proliferation. The protein TNF-alpha often connects indirectly with p38 through inflammatory pathways highlighting its involvement in these diseases.