Abcam, ab45910, Human CD31 ELISA Kit (PECAM1)

Size: 1 x 96Tests / 2 x 96Tests
Human CD31 ELISA Kit (PECAM1) is a Sandwich (quantitative) ELISA for the measurement of Human CD31 (PECAM1) in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:< 0.04 ng/mL,
Range:0.31 - 10 ng/mL,
Assay time:2h 45m,
Assay Platform:Microplate

Product details:
Abcam's CD31 (PECAM1) Human
in vitro
ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CD31 in Human serum, plasma, buffered solutions and cell culture medium
A monoclonal antibody specific for CD31 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD31 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD31 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD31 present in the samples.
This kit will recognize both endogenous and recombinant Human CD31.
Get results in 90 minutes with Human CD31 ELISA Kit (PECAM) (
ab190814
) from our SimpleStep ELISA
range.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C