Abcam, ab46022, Human IL-4 Receptor ELISA Kit

Size: 1 x 96Tests / 2 x 96Tests
Human IL-4 Receptor ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human IL-4 Receptor in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:< 11.5 pg/mL,
Range:31.25 - 1000 pg/mL,
Assay time:1h 45m,
Assay Platform:Microplate

Product details:
Abcam's Human IL-4 Receptor (IL-4R) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of soluble IL-4 receptor molecule (CD124) in Human serum, plasma, buffered solutions or cell culture medium.
A monoclonal antibody specific for IL-4R has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-4R concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-4R are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-4R present in the samples.
This kit will recognize both endogenous and recombinant Human IL-4R.
The standard is affinity purified.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C