Abcam, ab46063, Human IL-4 ELISA Kit High Sensitivity

Size: 1 x 96Tests
Human IL-4 ELISA Kit High Sensitivity is a Sandwich (quantitative) ELISA for the measurement of Human IL-4 High Sensitivity in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.31 pg/mL,
Range:0.31 - 10 pg/mL,
Assay time:3h,
Assay Platform:Microplate

Product details:
Abcam's IL-4 Human High Sensitivity
in vitro
ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-4 in Human serum, plasma, buffered solution and cell culture media.
A monoclonal antibody specific for IL-4 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-4 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-4 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-4 present in the samples.
This kit will recognize both endogenous and recombinant Human IL-4.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C