Abcam, ab46096, Mouse IL-2 ELISA Kit

Size: 1 x 96Tests / 2 x 96Tests
Mouse IL-2 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse IL-2 in Mouse in Cell Culture Media, Biofluids samples.
Key facts
Detection method:Colorimetric,
Sample types:Cell culture supernatant, Serum,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:< 3.4 pg/mL,
Range:15.6 - 500 pg/mL,
Assay time:3h 45m,
Assay Platform:Microplate

Product details:
Abcam's IL-2 mouse
in vitro
ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-2 in mouse serum, buffered solutions and cell culture medium.
A monoclonal antibody specific for IL-2 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-2 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-2 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-2 present in the samples.
This kit will recognize both endogenous and recombinant mouse IL-2.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C