Abcam, ab4641, Anti-NSP1 antibody [32D6]

Size: 250µL
Mouse Monoclonal NSP1 antibody. Suitable for WB, ICC/IF and reacts with Saccharomyces cerevisiae samples. Cited in 11 publications.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:32D6,
Isotype:IgG,
Carrier free:No,
Reacts with:Saccharomyces cerevisiae,
Applications:WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:This antibody was initially identified as directed against a nuclear pore complex antigen by immunofluorescence localization. A screen of a lgt11 expression library yielded a single positive clone carrying an insert bearing ~66% of the C-terminal portion of NSP1. To confirm that 32D6 possessed high affinity for NSP1, strain RS453, which expresses a shortened isoform of NSP1, was compared to the wildtype strain BJ5465. NSP1 in RS453 contains an internal deletion that removes the coding sequence for 6 FXFG repeats, which are not essential for function and encodes a protein that has been observed to migrate at approxmately 85 kDa on SDS gels. The predicted size of wildtype NSP1 is 86.5 kDa, but NSP1 has been observed to migrate on SDS gels at ~100 kDa. In our gels, the apparent molecular mass of wildtype NSP1 was 108 kDa, whereas the short isoform of NSP1 in RS453 migrated at 91 kDa. The detection of two protein bands of apparent sizes 108 kDa and 91 kDa in BJ5465 and RS453, respectively, demonstrated that 32D6 recognized the pore complex protein NSP1.

Product details:
This antibody is a useful marker of yeast nuclear pores.

Properties and Storage Information:
Form-Liquid, Purity-Tissue culture supernatant, Purification notes-Sterile filtered., Storage buffer-Preservative: 0.065% Sodium azideConstituents: Tissue culture supernatant, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The non-structural protein 1 (NSP1) is a protein encoded by the genome of coronaviruses including SARS-CoV-2 the virus responsible for COVID-19. It plays important mechanical roles in viral infection and replication. NSP1 weighing around 20 kDa acts by binding to the host's ribosomes where it inhibits host protein translation. This action shuts down the host's antiviral defense mechanisms aiding viral replication. NSP1 is expressed during coronavirus infections and has a role in disrupting host immune responses making it an important target in studying viral pathogenesis and immune evasion.
Biological function summary
NSP1 contributes to the suppression of host gene expression. It is part of a strategy employed by viruses to combat host immune defenses allowing them to replicate efficiently in host cells. By interfacing with the host's mRNA translation machinery NSP1 can selectively inhibit the production of proteins important for the immune response. This process involves interactions that lead to the degradation of host mRNAs preventing the synthesis of proteins needed to mount an effective defense against the virus.
Pathways
NSP1 is involved in intervening in host cellular pathways that control translation and immune signaling. It interacts with the ribosomal 40S subunit making it an essential element in the antiviral pathways specific to the interferon response. Proteins such as ribosomal proteins and other translation initiation factors are impacted by NSP1's presence which modulates their normal activities to favor viral protein synthesis. Its function in these pathways highlights NSP1's strategic importance in viral evasion tactics.
NSP1 plays a significant role in the pathogenesis of COVID-19 directly impacting how coronavirus diseases manifest and progress. The protein's interference with host cellular processes contributes to the severity of the illness given its role in dampening the immune response. NSP1 is also connected to proteins involved in the innate immune response such as interferon-stimulating genes that are part of the body's primary defense mechanisms. Understanding NSP1's function and interactions offers insights into therapeutic strategies that can mitigate the immune suppression caused by COVID-19 aiming to lessen disease burden and pathogenic outcomes.