Abcam, ab4729, Anti-Histone H3 (acetyl K27) antibody - ChIP Grade

Size: 100µg
Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729) is a rabbit polyclonal antibody detecting Histone H3 in Western Blot, IP, IHC-P, ICC/IF, ChIP, PepArr . Suitable for Cow, Human, Mouse, Rat, . - Over 2180 publications - Trusted since 2004
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Cow, Human,
Applications:IHC-P, PepArr, WB, ICC/IF, ChIPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.

Product details:
Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729) is a rabbit polyclonal antibody and is validated for use in ChIP, ICC/IF, IHC-P, PepArr and WB.
Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729) was first used in a scientific publication in 1975 and has been cited over 2188 times in peer reviewed journals. It's performance in ChIP and Western Blot in human and mouse samples is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729) has high sensitivity and specificity.
Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729) has 85 independent reviews from customers.
Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729) specifically detects Histone H3 Acetyl-K27 (UniProt ID: P68431; Molecular weight: 16kDa) and is sold in 100 µg selling sizes.
Top cited antibody for this target with >2500 citations and 70 five star abreviews. This antibody is useful in understanding H3K27ac's role in transcriptional activation and enhancer elements and is widely used as a marker in studies of post-translational modifications, embryonic genome activation and developmental biology. This antibody is crucial in cancer research and is widely used in studies of histone modifications involving histone acetyltransferases (HATs) and histone deacetylases (HDACs).

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Histone H3 is a core component of the nucleosome playing an important role in DNA packaging within eukaryotic cells. Often referred to as H3 it has a molecular weight of around 15 kDa. Histone H3 is expressed in the nucleus of nearly all eukaryotic cells where it interacts with DNA and other histone proteins to form chromatin structure. Scientists frequently study histone H3 using techniques like Western blotting and immunohistochemistry utilizing antibodies such as anti-histone or phospho-histone H3 to distinguish post-translational modifications.
Biological function summary
Histone H3 participates in chromatin remodeling and gene transcription regulation. As part of the nucleosome complex it influences DNA accessibility and subsequently gene expression. Through post-translational modifications including methylation and phosphorylation H3 modulates chromatin structure. Common modifications include mono- di- and tri-methylation at various lysine residues like K56 which affect transcriptional activation or repression. These modifications form a part of the histone code a framework for regulating chromatin dynamics and genetic information.
Pathways
Histone H3 modifications are integral to several signaling pathways that modulate gene expression and cellular responses. The histone code interpreted by other proteins such as transcription factors and chromatin remodelers contributes significantly to the regulation of the cell cycle and signal transduction pathways like the MAPK/ERK pathway. H3 interacts closely with regulatory proteins including other histones and transcription machinery to facilitate these complex processes.
Histone H3 modifications are associated with various health conditions including cancer and neurological disorders. Aberrations in histone H3 methylation are linked to oncogenic transformations with disruptions often found in cancer types like glioblastoma and breast cancer. The alterations in H3 also connect to Alzheimer's disease through interactions with other histone modifications and their impact on gene expression patterns critical for neural function. Understanding these modifications helps provide insights into disease mechanisms and potential therapeutic targets.