Abcam, ab4753, Anti-ATG8 antibody

Size: 250µg
Rabbit Polyclonal ATG8 antibody. Suitable for ELISA, WB and reacts with Saccharomyces cerevisiae samples. Cited in 12 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Saccharomyces cerevisiae S288C ATG8.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Saccharomyces cerevisiae,
Applications:WB, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Saccharomyces cerevisiae S288C ATG8.P38182

Properties and Storage Information:
Form-Liquid, Purity-IgG fraction, Purification technique-Ion exchange chromatography, Purification notes-Anti-Apg8 antibody, also known as Atg8, is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer., Storage buffer-Preservative: 0.01% Sodium azideConstituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The ATG8 protein also known as autophagy-related protein 8 weighs approximately 14 kDa and plays an important role in autophagy. It is expressed widely in eukaryotic cells including yeast plants and mammals. ATG8 engages in the conjugation with phospholipid phosphatidylethanolamine (PE) on the surface of autophagic vesicles. This conjugation allows the vesicles to elongate and form autophagosomes which are important for the engulfment of cellular components that need to be degraded.
Biological function summary
The ATG8 protein assists in the selective degradation of damaged organelles and proteins through autophagy. It is a member of the ATG ubiquitin-like protein family and works in collaboration with other autophagy proteins such as ATG3 and ATG7. These interactions form complexes necessary for the autophagic process. ATG8 acts as a marker for autophagosomes guiding cargo to be engulfed and recycled effectively.
Pathways
ATG8 functions within the autophagy pathway and the lysosome degradation pathway which are essential for cellular maintenance and nutrient recycling. In these pathways ATG8 interacts with proteins like mTOR and ULK1 that regulate autophagy. As a central player it connects upstream signaling events with downstream autophagic membrane dynamics facilitating cellular adaptation in response to nutrient availability and stress conditions.
Alterations in ATG8 function relate to neurodegenerative diseases such as Parkinson's disease and Huntington's disease. Dysregulation of autophagy where ATG8 plays a critical role impacts protein aggregation and cellular toxicity commonly seen in these conditions. Additionally it connects with proteins like p62 in these disorders as impaired protein clearance pathways influence disease progression. ATG8 involvement highlights its significance in maintaining neuronal health and potentially in therapeutic strategies targeting aberrant autophagic processes.