Abcam, ab48394, Anti-LC3B antibody - Autophagosome Marker

Size: 100µL
Anti-LC3B antibody - Autophagosome Marker (ab48394) is a rabbit polyclonal antibody detecting LC3B in Western Blot, IHC-P, ICC/IF . Suitable for Human, Mouse, Rat . - Over 700 publications - Trusted since 2007
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human MAP1LC3B. The exact immunogen used to generate this antibody is proprietary information.Q9GZQ8

Product details:
Anti-LC3B antibody - Autophagosome Marker (ab48394) is a rabbit polyclonal antibody and is validated for use in ICC/IF, IHC-P, WB in human samples.
Anti-LC3B antibody - Autophagosome Marker (ab48394) has been cited over 701 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-LC3B antibody - Autophagosome Marker (ab48394) has high sensitivity and specificity.
Anti-LC3B antibody - Autophagosome Marker (anti-MAP1LC3B antibody) (ab48394) has 32 independent reviews from customers.
Anti-LC3B antibody - Autophagosome Marker (ab48394) specifically detects LC3B (UniProt ID: Q9GZQ8; Molecular weight: 14kDa) and is sold in 100 µL selling sizes.
LC3B (MAP1LC3B) or Microtubule-associated protein 1 light chain 3 beta is a key marker of autophagy, a process that cancer cells often exploit for survival and growth. Elevated LC3B expression is commonly observed in various solid tumors and is associated with increased tumor proliferation and metastasis. LC3B facilitates the formation of autophagosomes, which help cancer cells manage stress and maintain cellular homeostasis.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
LC3B also known as microtubule-associated protein 1 light chain 3 beta plays an important role in autophagy a process that maintains cellular homeostasis. The LC3B protein with a molecular weight of approximately 14 kDa undergoes lipidation to form LC3-II which associates with autophagosomal membranes. This protein expresses ubiquitously in various tissues including liver muscle and brain. Researchers often detect LC3B using techniques like LC3B western blot and LC3B immunofluorescence due to its function as a marker indicating autophagy levels.
Biological function summary
LC3B contributes significantly to the formation and maturation of autophagosomes. LC3B part of the autophagy-related protein complex binds to autophagic membranes. During this process LC3-I converts to LC3-II a lipid-phosphatidylethanolamine conjugate essential for autophagosome membrane expansion and closure. This mechanism helps remove damaged organelles and misfolded proteins from cells therefore contributing to cellular quality control.
Pathways
LC3B integrates into the autophagy pathway which is critical for cellular adaptive responses to stress. The mammalian target of rapamycin (mTOR) pathway regulates autophagy where mTOR inhibition activates LC3B promoting autophagosome formation. Moreover LC3B operates alongside other proteins like Beclin-1 and ULK1 facilitating the initiation and progression of autophagy under nutrient starvation conditions. These interactions highlight LC3's role in cellular energy balance and survival mechanisms.
LC3B connects with conditions such as cancer and neurodegenerative diseases. Altered autophagy levels mediated by LC3B often associate with tumorigenesis where its dysregulation can affect cancer progression. Furthermore LC3B also links to neurodegenerative diseases like Alzheimer's where impaired autophagy disrupts neuronal function. LC3B interacts with proteins such as p62/SQSTM1 which affects protein aggregate clearance a critical factor in neurodegenerative pathology.