Abcam, ab48626, Anti-Thrombin antibody

Size: 150µg
Rabbit Polyclonal Thrombin antibody. Suitable for IP, ELISA, WB, IHC-P, RIA, ICC/IF, EIA and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Native Full Length Protein corresponding to Human F2.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ELISA, IHC-P, RIA, ICC/IF, EIA, WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Native Full Length Protein corresponding to Human F2.P00734,
Specificity:The antibody will recognize prothrombin and thrombin

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Thrombin also known as Factor II or thrombin protein is a 36 kDa serine protease essential for blood coagulation. It is produced in the liver as prothrombin and activated in the coagulation cascade. Its expression occurs extensively in the liver where it plays an important role in converting soluble fibrinogen into insoluble fibrin forming blood clots. Thrombin also activates various other coagulation factors including Factors V VIII and XI amplifying the coagulation response. Biotinylated thrombin variations help in detection and research applications.
Biological function summary
Thrombin modulates several physiological processes beyond clot formation. It serves as a signaling molecule interacting with protease-activated receptors (PARs) to influence cell functions including proliferation migration and apoptosis. Thrombin forms part of the prothrombinase complex comprised of prothrombin activated Factor X (Xa) and Factor V on phospholipid surfaces. This complex is critical for thrombin generation during the clotting cascade. Thrombin's activity extends to involvement in wound healing and inflammation regulation.
Pathways
Thrombin significantly participates in the coagulation and fibrinolytic pathways. It not only converts fibrinogen to fibrin in the coagulation pathway but also activates inhibitors like antithrombin which regulates thrombin and other protease activities. Thrombin's interaction with fibrinolysis where tPA and plasminogen are substrates integrates clot disintegration processes. Thrombin's connectivity with proteins such as Factor VII Protein C and antithrombin outlines its diverse role in maintaining hemostatic balance.
Thrombin associates with conditions such as venous thromboembolism and disseminated intravascular coagulation (DIC). Dysregulation of thrombin leads to excessive clotting contributing to thromboembolic diseases. In DIC uncontrolled thrombin activity results in systemic coagulation consuming clotting factors and increasing bleeding risk. Its function alongside proteins like Factor V Leiden and antithrombin deficiencies aggravate thrombotic conditions. Research into thrombin inhibitors aids in developing therapeutic strategies for these disorders.