Abcam, ab50038, Recombinant human TGF beta 1 protein (Active)

Size: 10µg
Recombinant human TGF beta 1 protein (Active) is a Human Full Length protein, in the 279 to 390 aa range, expressed in CHO cells, with >98%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, HPLC.
Key facts
Purity:>98% SDS-PAGE,
Endotoxin level:< 1 EU/µg,
Expression system:CHO cells,
Tags:Tag free,
Applications:HPLC, SDS-PAGE, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:The ED50, as determined by TGF beta 1's ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells, is ≤ 0.05 ng/ml, corresponding to a specific activity of >2 x 107 units/mg,
Accession:P01137,
Animal free:No,
Carrier free:No,
Species:Human,
Reconstitution:Centrifuge the vial prior to opening. For lot GR18648 Reconstitute in 10mM Citric Acid, pH 3.0 to 0.1-1.0 mg/ml. For all other lots reconstitute in water to a concentration of 0.1- 1.0 mg/ml. Do not vortex. If you have any questions regaring the reconstitution of ab50038 please contact scientific support.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA), Working aliquots stored with a carrier protein are stable for at least 3 months at -20°C to -80°C.