Abcam, ab50242, Recombinant human Apolipoprotein E3

Size: 500µg
Recombinant human Apolipoprotein E3 is a Human Full Length protein with >=90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE. The predicted molecular weight of ab50242 protein is 34.4 kDa. - Save time and ensure accurate results - use our Apolipoprotein E3 (ApoE3) protein as a control - Optimal protein bioactivity and stability
Key facts
Purity:>90% SDS-PAGE,
Endotoxin level:< 1 EU/µg,
Expression system:Escherichia coli,
Tags:Tag free,
Applications:SDS-PAGE, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Determined by its ability to stimulate the proliferation of human SH-SY5Y cells.,
Accession:P02649,
Animal free:No,
Carrier free:No,
Species:Human,
Reconstitution:Reconstitute in 20mM Sodium Phosphate, pH 7.8 + 0.5mM DTT to 1.0 mg/ml. Store at 2°C to 8°C for up to 1 week, or Prepare for extended storage at -20°C to -80°C for up to 3 months.

Product details:
Ensure the validity of your result using our bioactive recombinant human Apolipoprotein E3 (ApoE3) protein ab50242 as a positive control in SDS-PAGE.
Check out our protein gel staining guide for SDS-PAGE
Check out of western blot protocol for more information

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Apolipoprotein E3 also known as ApoE3 or Apo E3 is an important molecule in lipid metabolism. The protein has a molecular weight of approximately 34 kDa. It is mainly expressed in the liver though significant amounts are produced by astrocytes and microglia in the central nervous system. ApoE3 is one of three major isoforms of apolipoprotein E and it plays an important role in the transport and clearance of lipids especially cholesterol by binding to lipoproteins and cellular receptors.
Biological function summary
The protein participates in lipid transport and homeostasis. ApoE3 binds to lipoprotein particles forming a complex that is essential for the proper transport of lipids in the bloodstream. This binding mechanism facilitates the uptake of lipoprotein particles by cells through receptor-mediated endocytosis. ApoE3 interacts closely with other lipid-associated proteins and receptor molecules enabling the efficient delivery of cholesterol and phospholipids to many tissues which is critical for maintaining cellular membrane integrity and other lipid-based biological functions.
Pathways
ApoE3 is an important player in the cholesterol metabolism and reverse cholesterol transport pathways. The protein interacts with LDL and HDL receptors which regulate cholesterol levels in the plasma. ApoE3's role in the hepatic clearance of chylomicrons and other lipoprotein remnants is significant for maintaining lipid balance. Additionally it cooperates with proteins like LDLR (Low-Density Lipoprotein Receptor) and LRP1 (LDL Receptor Related Protein 1) in orchestrating these pathways.
Research has shown that ApoE3 is less associated with adverse conditions than other isoforms like ApoE4. However disruptions in ApoE3 function or expression can contribute to dyslipidemia and cardiovascular diseases. In Alzheimer's disease the protein's isoforms particularly ApoE4 have varying implications but understanding ApoE3's interactions can provide insights into lipid-related pathology. ApoE3's connection with LDLR and its modulation of cholesterol levels underline its potential therapeutic role in managing such disorders.