Abcam, ab5076, Anti-Iba1 antibody - Microglia marker

Size: 100µg
Anti-Iba1 antibody (ab5076) is a goat polyclonal antibody detecting Iba1 in Western Blot, IHC-P . Suitable for Human, Rat . - Over 1350 publications - Trusted since 2003
Key facts
Host species:Goat,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Rat, Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human AIF1 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.P55008,
Specificity:ab5076 is expected to recognize the isoforms represented by NP_001614 and NP_116573 but not NP_004838.Although we received several excellent papers on ab5076 working with rat IHC-P, rat is not a batch tested species for IHC-P and we cannot guarantee.

Product details:
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Microglia markers guide
Anti-Iba1 antibody (ab5076) is a Goat Polyclonal antibody and is validated for use in IHC-P, WB in human, rat samples.
Anti-Iba1 antibody (ab5076) has been cited over 1354 times in peer reviewed journals and is trusted by the scientific community.
Abcams high quality validation processes ensure Anti-Iba1 antibody (ab5076) has high sensitivity and specificity.
Anti-Iba1 antibody (ab5076) has 100 independent reviews from customers.
Anti-Iba1 antibody (ab5076) specifically detects Iba1 (UniProt ID: P55008; Molecular weight: 17kDa) and is sold in 100 ug selling sizes.
Iba1 (Ionized calcium-binding adapter molecule 1; allograft inflammatory factor 1, AIF1) is a prominent microglial marker extensively used in neuroscience research. Its expression increases in various neurodegenerative conditions, including Alzheimer's disease (AD), indicating microglial activation and neuroinflammation. Researchers utilize Iba1 in stainings to assess microglial response and activity in different models, making it essential for understanding inflammatory processes in the brain. Changes in Iba1 expression provide valuable insights into neuroinflammation, highlighting its importance in studying diseases like AD.
The Iba1 antibody (ab5076) is commonly used as a marker of microglia activation in staining and immunohistochemistry, given that ionized calcium binding adaptor molecule 1 (Iba1) is a microglia/macrophage-specific calcium-binding protein with actin-bundling activity that participates in membrane ruffling and phagocytosis in activated microglia.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide., Storage buffer-pH: 7.3Preservative: 0.02% Sodium azideConstituents: Tris buffered saline, 0.5% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Iba1 also known as allograft inflammatory factor 1 (AIF1) is a protein with a molecular weight of approximately 17 kDa. It is widely expressed in microglia which are the resident immune cells of the central nervous system (CNS). Iba1 plays a mechanical role in actin-binding and modulating cytoskeleton dynamics which affects cell shape and motility. It is also found in other myeloid-derived cells including macrophages and monocytes where it influences cellular processes linked to inflammation and immune responses.
Biological function summary
The involvement of Iba1 in actin polymerization impacts microglia's ability to perform phagocytosis and cytokine production. Iba1 does not appear to be part of any large protein complex but interacts directly with actin filaments to facilitate cytoskeletal reorganization. This function enables the cells to move and respond efficiently to tissue damage or pathogens in the CNS. It plays an important role in supporting cellular changes associated with immune surveillance and neuroinflammatory responses.
Pathways
Microglial activation relies heavily on Iba1 for executing acts linked with neuroinflammation. Iba1 engages in the NF-kB signaling pathway significant in managing inflammatory responses. It interacts with proteins such as MyD88 and IRAK4 which aid in transmitting signals from toll-like receptors. These pathways enable changes in gene expression critical for microglial response to various stimuli. Furthermore Iba1 also contributes to the PI3K/Akt pathway where it influences cell survival and proliferation interacting with proteins like Akt1 and PTEN.
Iba1 has implications in neurodegenerative diseases notably Alzheimer's disease and multiple sclerosis. In Alzheimer's disease increased expression of Iba1 is observed in activated microglia around amyloid plaques suggesting a role in plaque clearance and neuroinflammation. The protein relates to amyloid precursor protein (APP) as microglia respond to abnormal accumulations. In multiple sclerosis Iba1 expression marks activated microglia involved in demyelination processes where it associates with proteins like myelin basic protein (MBP) highlighting its role in the disease's pathology.