Abcam, ab51129, Anti-14-3-3 zeta antibody

Size: 100µg
Rabbit Polyclonal 1433Z antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 38 publications. Immunogen corresponding to Synthetic Peptide within Human 14-3-3 protein zeta/delta.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human 14-3-3 protein zeta/delta. The exact immunogen used to generate this antibody is proprietary information.P63104,
Specificity:ab51129 detects endogenous levels of total 14-3-3 zeta protein.Cross reactivity of ab51129 antibody with other member of the 14-3-3 family:-92% identities with 14-3-3 beta/alpha, gamma and eta-77% identities with 14-3-3 teta-69% identities with 14-3-3 epsilon-62% identities with 14-3-3 sigma

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The 14-3-3 zeta protein also known as 14-3-3 protein zeta/delta and 1433 protein belongs to the 14-3-3 protein family. These proteins have a molecular weight of around 28-33 kDa. 14-3-3 zeta proteins are expressed across various human tissues including the brain heart and muscles. Mechanically the 14-3-3 zeta protein interacts with different phosphorylated serine and threonine residues on its target proteins. This binding modulates the target proteins' conformation and activity influencing their function in cellular signaling and regulation.
Biological function summary
The 14-3-3 zeta protein plays an important role in several cellular processes. It acts as an adapter in signal transduction pathways and cell cycle control. Additionally the 14-3-3 zeta protein forms complexes with other proteins to regulate apoptosis and stress responses. By binding to various partners this protein influences the distribution and localization of proteins within the cell affecting diverse functions such as metabolism and cell growth.
Pathways
The 14-3-3 zeta protein participates actively in two primary pathways: the MAPK/ERK and PI3K/AKT pathways. Within the MAPK/ERK pathway it influences cellular differentiation and proliferation interacting closely with proteins like Raf and MEK. In the PI3K/AKT pathway 14-3-3 zeta affects cell survival working alongside proteins such as AKT and Bad. These interactions make 14-3-3 zeta a significant player in the regulation of cellular processes.
14-3-3 zeta protein has been connected with neurodegenerative diseases and cancer. Altered expression or function of this protein has been observed in conditions like Alzheimer's disease where it interacts with tau protein and in various cancers where 14-3-3 zeta impacts tumor progression by modulating the function of proteins like p53 and Rb. Understanding these interactions helps in exploring therapeutic strategies for disease intervention.