Abcam, ab5131, Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody

Size: 50µg
Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (ab5131) is a rabbit polyclonal antibody for Western Blot, IP, IHC, ICC/IF, ELISA, ChIP in Arabidopsis, C.elegans, Drosophila, Human, Mouse, Rat, S.cerevisiae, S.pombe, Xenopus, Zebrafish . - Over 440 publications - Trusted since 2005
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Zebrafish, Mouse, Saccharomyces cerevisiae, Arabidopsis thaliana, Schizosaccharomyces pombe, Xenopus laevis, Rat, Caenorhabditis elegans, Drosophila melanogaster,
Applications:IHC-P, ChIP, IHC-Fr, IP, ELISA, WB, IHC - Wmt, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:This product was produced with the following immunogens:The exact immunogen used to generate this antibody is proprietary information.The exact immunogen used to generate this antibody is proprietary information.The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody recognises the phosphorylated serine found in the amino acid 5 position of the C-terminal domain repeat YSPTSPS of RNA polymerase II. From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.

Product details:
Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (ab5131) is a rabbit polyclonal antibody and is validated for use in ChIP, ELISA, ICC/IF, IHC - Wmt, IHC-Fr, IHC-P, IP, WB.
Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (ab5131) has been cited over 444 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (ab5131) has high sensitivity and specificity.
Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (ab5131) has 48 independent reviews from customers.
Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (ab5131) specifically detects RNA polymerase II CTD repeat YSPTSPS Phospho-S5 (UniProt ID: P24928; Molecular weight: 217kDa) and is sold in 50 µg selling sizes.
RNA polymerase II CTD repeat YSPTSPS (phospho S5) is crucial for transcription regulation and gene expression. Research indicates that phosphorylation at serine 5 is essential for the transition from transcription initiation to elongation, impacting various cellular processes and disease states. Phosphorylation of RNA polymerase II at serine 5 is critical for proper mRNA synthesis and processing. Abnormal phosphorylation patterns are linked to transcriptional dysregulation in cancer, pediatric gliomas, chondroblastomas, and neurodevelopmental syndromes. Studying these modifications can provide insights into potential therapeutic targets for various conditions.
Phosphorylation of RNA polymerase II's largest subunit C-terminal domain (CTD) is a key event during mRNA metabolism.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RNA polymerase II CTD repeat YSPTSPS also known as the C-terminal domain of RNA polymerase II is a critical component of the RNA polymerase II enzyme commonly referred to as pol II. This domain is characterized by the repetitive sequence YSPTSPS which plays a significant role in the regulation of transcription. The mass of RNA polymerase II including its CTD varies but is essential for its function in gene expression. RNA polymerase II with the CTD is expressed in the nucleus of eukaryotic cells where it orchestrates the transcription of DNA into mRNA.
Biological function summary
RNA polymerase II CTD repeat YSPTSPS is essential for the transcription progression from initiation to termination. It is part of the large RNA polymerase II complex interacting with various transcription factors and enzymes necessary for RNA processing. The phosphorylation state of the CTD particularly on serine residues regulates interactions with splicing machinery and other RNA processing factors. This modulation ensures the coupling between transcription and RNA processing events controlling mRNA synthesis and maturation.
Pathways
RNA polymerase II CTD repeat YSPTSPS is important in the mRNA synthesis pathway specifically in transcriptional regulation and processing of nascent RNA transcripts. It interacts with proteins such as the transcription factors TFIIH and TFIIB which aid in promoter recognition and open complex formation. The CTD's dynamic phosphorylation pattern allows integration into multiple cellular pathways most importantly connecting transcription with RNA splicing and transport pathways.
Abnormal function or mutations in RNA polymerase II CTD repeat YSPTSPS associate with diseases such as transcription-related syndromes and certain cancers. Deficient CTD phosphorylation can lead to improper mRNA processing resulting in neural developmental disorders. Additionally its interaction with proteins like CDK7 which phosphorylates the CTD links it to tumors where transcriptional dysregulation is a hallmark. Understanding the CTD's role in these diseases provides insight into therapeutic targets and strategies for intervention.