Abcam, ab51608, Anti-HIF-1 alpha antibody [EP1215Y]

Size: 100µL / 1mL
Anti-HIF-1 alpha antibody [EP1215Y] (ab51608) is a rabbit monoclonal antibody detecting HIF-1 alpha in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF . Suitable for Human . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 340 publications - Trusted since 2007
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP1215Y,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, ChIC/CUT&RUN-seq, IP, ICC/IF, Flow Cyt (Intra), IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody recognizes HIF-1-alpha.For mouse specific Hif-1-alpha rabbit monoclonal antibody, please see ab179483 (clone ID: EPR16897). ab179483 has been confirmed for mouse samples in WB.HIF-1 alpha expression is absent in most normal tissues (PMID: 12128120, 24835245, 11689469, 20217131).

Product details:
Anti-HIF-1 alpha antibody [EP1215Y] (ab51608) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ChIC/CUT&RUN-seq, Flow Cyt (Intra), ICC/IF, IHC-P, IP, WB in human samples.
Anti-HIF-1 alpha antibody [EP1215Y] (ab51608) has been cited over 340 times in peer reviewed journals and is trusted by the scientific community.
Abcams high quality manufacturing and validation processes ensure Anti-HIF-1 alpha antibody [EP1215Y] (ab51608) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
The specificity of Anti-HIF-1 alpha antibody [EP1215Y] (ab51608) has been confirmed by testing in knockout samples.
Anti-HIF-1 alpha antibody [EP1215Y] (ab51608) has 12 independent reviews from customers.
Anti-HIF-1 alpha antibody [EP1215Y] (ab51608) specifically detects HIF-1 alpha (UniProt ID: Q16665; Molecular weight: 93kDa) and is sold in 100 uL and 1 mL selling sizes.
Conjugation-ready, carrier free format available for antibody clone EP1215Y -
ab2173
Antibody clone EP1215Y is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 647 (
ab19569
HIF-1 alpha, also known as hypoxia-inducible factor 1-alpha (HIF1A), is a critical regulator of cellular response to low oxygen levels. HIF1A is a transcription factor commonly referred to as a "master regulator of the hypoxic response" for its central role in the regulation of cellular adaptations to hypoxia. Hypoxia contributes to the pathophysiology of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. A highly specific HIF-1 alpha antibody, essential for studying hypoxia-inducible factors and oxygen homeostasis. This antibody is crucial in tumor hypoxia research, particularly in understanding cancer progression and angiogenesis. It is widely used in studies of metastasis and HIF-1 alpha inhibitors. The HIF1A molecular weight is approximately 120 kDa, and detecting it accurately is essential for understanding its role in the tumor microenvironment. Elevated HIF-1 alpha levels are linked to tumor progression, angiogenesis, and metastasis. Monitoring HIF-1 alpha can provide valuable insights into cancer biology and potential therapeutic targets. This antibody is also validated for CUT&RUN-seq, which is a key application to map protein-DNA interactions on a genome-wide scale using NGS.
For Mouse specific Hif-1-alpha rabbit monoclonal antibody, please see
ab179483 (clone ID: EPR16897)
ab179483
has been confirmed for Mouse sample in WB.
We have mixed customer feedback towards the rat specificity so we are unable to confirm and guarantee its performance with rat samples. Please contact technical team for more information.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HIF-1 alpha also known as hypoxia-inducible factor 1-alpha is a transcription factor critical in cellular response to low oxygen levels. Its molecular weight usually ranges from 93 to 120 kDa. You can find HIF-1 alpha expressed in tissues throughout the body but its expression significantly increases under hypoxic conditions. Researchers often use the HIF-1a ELISA to measure its expression levels. HIF-1 alpha forms a complex with other proteins to perform its functions effectively.
Biological function summary
HIF-1 alpha regulates gene expression in response to hypoxic conditions in cells. It forms a complex with HIF-1 beta to activate transcription of various genes involved in energy metabolism angiogenesis and erythropoiesis. HIF-1 alpha enables cells to adapt to reduced oxygen availability allowing for cellular survival and function under stress. It plays an important role in promoting the expression of genes like VEGF and EPO which are important for vascular and red blood cell development respectively.
Pathways
HIF-1 alpha plays an integral role in the hypoxia signaling pathway and the glycolytic pathway. In the hypoxia signaling pathway HIF-1 alpha partners with VHL (Von Hippel-Lindau) protein that regulates its degradation under normal oxygen conditions. When oxygen levels drop HIF-1 alpha avoids degradation stabilizes and translocates into the nucleus to initiate transcription of hypoxia-responsive genes. The glycolytic pathway involvement highlights its function in adapting energy production under hypoxic conditions through collaboration with enzymes and transporters associated with glycolysis.
HIF-1 alpha has been implicated in cancer and ischemic diseases. Its role in promoting angiogenesis and metabolic adaptation makes it a contributor to tumor growth and survival collaborating with oncogenes such as c-Myc. In ischemic diseases like stroke or myocardial infarction HIF-1 alpha's ability to induce protective responses can mitigate tissue damage through regulation of survival pathways. Understanding these interactions helps in the development of therapeutic strategies targeting HIF-1 alpha in disease contexts.