Abcam, ab51862, Anti-CD69 antibody [FN50]

Size: 100µg
Mouse Monoclonal CD69 antibody. Suitable for Flow Cyt, IHC-Fr and reacts with Human samples. Cited in 4 publications.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:FN50,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:No,
Reacts with:Human,
Applications:IHC-Fr, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Product details:
Original vial: 1 year at 4° - 8°C Stock solution or aliquots: 1 year at -20°C. Avoid repeated thawing and freezing.

Properties and Storage Information:
Form-Lyophilized, Reconstitution-Reconstitute in 0.5 mL of water, Purification notes-Purified from tissue culture supernatant by affinity chromatography., Storage buffer-pH: 7.2 Preservative: 0.09% Sodium azide Constituents: Water, BSA, PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-Up to 12 months, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CD69 also known as Activation-Induced C-type Lectin or Early T-cell Activation Antigen is a type II transmembrane protein with a mass of approximately 28-32 kDa. It is expressed mainly on activated leukocytes including T cells B cells and natural killer (NK) cells. Expression occurs rapidly after cell activation making CD69 a robust early marker used in flow cytometry analysis like with CD69 FITC or CD69 PE conjugates to study immune cell activation. The mouse version of CD69 serves as an important model for understanding its function in human biology.
Biological function summary
CD69 functions as a signaling molecule during the immune response influencing both cell proliferation and cytokine production. It is a member of the C-type lectin superfamily and operates as a homodimer on the cell surface. CD69 helps modulate the migration of T cells to inflammatory sites by affecting sphingosine-1-phosphate receptor 1 (S1P1) downregulation directing their tissue retention and primary response roles.
Pathways
The protein plays an important role in the regulation of the NF-kB and JAK-STAT signaling pathways. These are important for immune cell communication and response and CD69 interacts with proteins like the IL-2 receptor complex in these pathways. Additionally its cross-linking can lead to altered expression of cytokines and chemokines impacting overall immune function.
Altered CD69 expression links to conditions like autoimmune diseases and chronic inflammation. Its involvement in rheumatoid arthritis shows how CD69 may regulate abnormal immune responses. Furthermore the modulation of CD69 expression can be associated with cancer progression highlighting connections to proteins like FN50 and FN61 which participate in tumor immunity and metastasis control.