Abcam, ab51997, Anti-Histone H4 (acetyl K5) antibody [EP1000Y] - ChIP Grade

Size: 20µL / 100µL / 1mL
Anti-Histone H4 (acetyl K5) antibody [EP1000Y] - ChIP Grade (ab51997) is a rabbit monoclonal antibody detecting Histone H4 in Western Blot, IP, IHC-P, ICC/IF, ELISA, ChIP . Suitable for Human, Mouse, Rat, . - Biophysical QC for unrivalled batch-batch consistency - Over 100 publications - Trusted since 2007
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP1000Y,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, IP, WB, ELISA, ICC/IF, ChIPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody cross-reacts with H4K8ac and H4unmod peptides in peptide array assay. However, the cross-reactivity is minimal in ELISA when using optimized peptide concentration (images on website). Further optimization is needed to minimize the cross-reactivity in assays similar to peptide array.

Product details:
What is this antibody validated in?
Anti-Histone H4 (acetyl K5) antibody [EP1000Y] - ChIP Grade (ab51997) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA, ChIP in Human, Mouse, Rat, samples.
What is the molecular weight of Histone H4?
Anti-Histone H4 (acetyl K5) [EP1000Y] - ChIP Grade (ab51997) specifically detects a band for Histone H4 (UniProt: P62805) at a molecular weight of 11kDa.
Trusted by the scientific community
Anti-Histone H4 (acetyl K5) [EP1000Y] - ChIP Grade (ab51997) was first used in a scientific publication in 2007 and has been cited over 100 times in peer-reviewed journals.
Reviewed by scientists
Anti-Histone H4 (acetyl K5) [EP1000Y] - ChIP Grade (ab51997) has over 5 independent reviews from customers.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
Discover our selection of trial-size antibodies
Other related products
We have a range of other formats of antibody clone [EP1000Y] also available for your convenience: ab51997, Biotin -
ab202886
, Carrier free -
ab232507
, Alexa Fluor® 488 -
ab313346
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle