Abcam, ab52451, PE Anti-HLA Class I antibody [MEM-147]

Size: 100µg
Mouse Monoclonal HLA A antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing HLA-A protein.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:MEM-147,
Isotype:IgG1,
Conjugation:PE,
Excitation/Emission:Ex: 480;565nm, Em: 578nm,
Carrier free:No,
Reacts with:Human,
Applications:Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Cell preparation containing HLA-A protein. The exact immunogen used to generate this antibody is proprietary information.P04439,
Specificity:ab5451 recognises all human classical MHC Class I molecules in nativecell-surface forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human cell types.ab52451 is positive in Western blotting (non-reducing conditions) only with most HLA-B and HLA-C molecules, but not HLA-A.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-ab52451 was purified by Protein G prior to conjugation. After conjugation, it was purified by site exclusion chromatography. Purity >95% by SDS-PAGE., Storage buffer-pH: 7.4Preservative: 0.097% Sodium azideConstituents: 0.2% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C