Abcam, ab52452, FITC Anti-Glycophorin A + B antibody [HIR2]

Size: 50Test
Mouse Monoclonal Glycophorin A antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human GYPA.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:HIR2,
Isotype:IgG2b,
Conjugation:FITC,
Excitation/Emission:Ex: 495nm, Em: 519nm,
Carrier free:No,
Reacts with:Human,
Applications:Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human GYPA. The exact immunogen used to generate this antibody is proprietary information.P02724,
Specificity:ab52452 recognises N-terminal, homologous portion of glycophorins A (GPA) and B (GPB) which are single-pass membrane sialoglycoproteins expressed on red blood cells and erythroid precursor cells.

Product details:
ab52452 agglutinates untreated RBCs but it failes to agglutinate papain-treated cells, binds to GPA, but weakly to GPB. The antibody is useful in erythroid cell development studies, because HIR2 antigen is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells (mature erythrocytes are characteristically CD235a positive and CD45 and CD71 negative).

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Prior to conjugation, ab52452 was purified by Protein G affinity chromatography. After conjugation, it was purified by site exclusion chromatography. Purity >95% by SDS-PAGE., Storage buffer-pH: 7.4Preservative: 0.097% Sodium azideConstituents: PBS, 0.2% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Glycophorin A and Glycophorin B are well-known sialoglycoproteins located on the surface of human erythrocytes. They are often referenced by alternate names like GYPHE for Glycophorin E and Glycophorin A+B for the combination of both proteins. Glycophorin A which can be labeled with FITC for detection has a molecular mass of approximately 31.3 kDa while Glycophorin B has a similar but slightly smaller mass. These proteins are essential components of the erythrocyte membrane contributing significantly to the negative charge and structural stability of red blood cells.
Biological function summary
Glycophorins A and B interact with other proteins in the erythrocyte membrane influencing the cell's architecture and the stability of the cytoskeleton. These glycophorins associate with CD235a known for its role in red blood cell recognition and interactions. Glycophorin A participates in the formation of a protein complex with other membrane proteins contributing to the antigenic properties of red blood cells which is important in blood transfusion biology.
Pathways
Glycophorin A and B participate in several key biological processes such as membrane organization and signal transduction pathways. They are involved in the MNS blood group system and interact with proteins like band 3 which is a critical component of the erythrocyte's anion exchange mechanism. This interaction helps facilitate ion transport and gas exchange in red blood cells.
Glycophorins A and B have associations with malaria and autoimmune hemolytic anemia. The malaria parasite Plasmodium falciparum interacts with these glycophorins to invade red blood cells. Changes in the glycan composition of glycophorin A contribute to the pathophysiology of certain autoimmune disorders potentially linking to immune recognition proteins. Managing these interactions and modifications can be important in designing therapeutic interventions for these conditions.