Abcam, ab52816, Anti-Cytokeratin 15 antibody [EPR1614Y]

Size: 20µL / 100µL / 1mL
Anti-Cytokeratin 15 antibody [EPR1614Y] (ab52816) is a rabbit monoclonal antibody detecting Cytokeratin 15 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF . Suitable for Human, Mouse . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 60 publications - Trusted since 2007
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR1614Y,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:Flow Cyt (Intra), IHC-P, ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-Cytokeratin 15 antibody [EPR1614Y] (ab52816) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse samples.
What is the molecular weight of Cytokeratin 15?
Anti-Cytokeratin 15 [EPR1614Y] (ab52816) specifically detects a band for Cytokeratin 15 (UniProt: P19012) at a molecular weight of 45kDa.
Trusted by the scientific community
Anti-Cytokeratin 15 [EPR1614Y] (ab52816) was first used in a scientific publication in 2007 and has been cited over 60 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
Discover our selection of trial-size antibodies
Specificity confirmed
The specificity of Anti-Cytokeratin 15 antibody [EPR1614Y] (ab52816) has been confirmed by Western blot testing in KRT15 Knockout A431 cells.
Other related products
We have a range of other formats of antibody clone [EPR1614Y] also available for your convenience: ab52816, Alexa Fluor® 488 -
ab194065
, Alexa Fluor® 647 -
ab194066
, Alexa Fluor® 555 -
ab214393
, Carrier free -
ab239850
, PE -
ab305710
, APC -
ab305711
, HRP -
ab305712
, Alkaline Phosphatase -
ab308967
, Alexa Fluor® 594 -
ab310739
, Alexa Fluor® 568 -
ab312759
, Alexa Fluor® 750 -
ab321667
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.05% Sodium azideConstituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Cytokeratin 15 often abbreviated as CK15 bsa-15 or keratin 15 is an intermediate filament protein known for its role in the structural integrity of epithelial cells. It weighs approximately 50 kilodaltons. This protein is mainly expressed in the basal layer of stratified squamous epithelia such as the epidermis and the hair follicle. CK15 is an essential part of the cytoskeleton contributing to cell shape and mechanical resilience.
Biological function summary
CK15 plays a significant role in maintaining epithelial stem cell populations. It is part of the keratin protein family where it forms a complex network with other cytokeratins to support epithelial cell stability. CK15 has been shown to mark epithelial progenitor cells hinting at its role in tissue regeneration and repair. Its expression levels often reflect the cellular origin and differentiation state particularly in the skin and associated structures.
Pathways
CK15 is involved in pathways related to epithelial cell differentiation and homeostasis. It interacts within the cytoskeletal network alongside other cytokeratins like keratin 5. CK15 contributes to pathways that regulate cell proliferation and differentiation. It participates in the wound healing process where keratin 6 and keratin 17 also play important roles. This involvement allows it to impact how epithelial cells respond to and repair after damage.
CK15 has been associated with skin disorders and certain types of cancers. Its expression is often altered in squamous cell carcinoma and basal cell carcinoma where abnormal CK15 levels can influence tumor behavior. Additionally changes in CK15 expression are linked to alopecia relating it to proteins like keratin 5 and keratin 8 which have similar expression patterns in hair follicles. Understanding CK15's role may aid in developing biomarkers for diagnosis and therapeutic targets.