Abcam, ab52865, Anti-Musashi 1 / Msi1 antibody [EP1302]

Size: 100µL / 1mL
Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865) is a rabbit monoclonal antibody detecting Musashi 1 / Msi1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF . Suitable for Chicken, Human, Mouse, Quail . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 50 publications - Trusted since 2007
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP1302,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Chicken, Human, Quail,
Applications:Flow Cyt (Intra), ICC/IF, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Several customers have found that this antibody gives good results in mouse and rat however in our hands, we cannot obtain positive results. This antibody is therefore no longer covered by our Abpromise guarantee for use in mouse or rat.

Product details:
What is this antibody validated in?
Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Chicken, Human, Mouse, Quail samples.
What is the molecular weight of Musashi 1 / Msi1?
Anti-Musashi 1 / Msi1 [EP1302] (ab52865) specifically detects a band for Musashi 1 / Msi1 (UniProt: O43347) at a molecular weight of 39kDa.
Trusted by the scientific community
Anti-Musashi 1 / Msi1 [EP1302] (ab52865) was first used in a scientific publication in 2007 and has been cited over 50 times in peer-reviewed journals.
Reviewed by scientists
Anti-Musashi 1 / Msi1 [EP1302] (ab52865) has over 15 independent reviews from customers.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
Discover our selection of trial-size antibodies
Specificity confirmed
The specificity of Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865) has been confirmed by Western blot testing in MSI1 Knockout HAP1 cells.
Other related products
We have a range of other formats of antibody clone [EP1302] also available for your convenience: ab52865, Alexa Fluor® 488 -
ab199781
, Alexa Fluor® 647 -
ab199838
, PE -
ab210418
, Carrier free -
ab221797
, APC -
ab310871
, Alexa Fluor® 594 -
ab311702
, Alexa Fluor® 568 -
ab312977
, Alexa Fluor® 555 -
ab313185
, Alexa Fluor® 750 -
ab321668
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Shipping conditions update: ambient shipping
This product will be delivered at ambient temperature instead of chilled – this is by design. Extensive stability testing confirmed that our products are suitable for shipment under ambient conditions and maintain expected quality.
Why the change?
It’s part of our commitment to more sustainable packaging solutions, with ambient deliveries using eco-friendly materials such as recyclable cardboard instead of polystyrene.
What you need to know
Ambient shipments come clearly marked on the delivery note.
No ice will be included in ambient shipments, but mixed orders (ambient and cold-chain items) will still arrive with ice packs to protect temperature-sensitive products.
Warranty coverage remains fully valid, aligned with our validated shipping method.
Please store the product as per the datasheet instructions upon receipt.
Find out more -
https://www.abcam.com/en-us/support/shipping-storage-support/ambient-shipping

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Conditional Ambient, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Musashi 1 also known as Msi1 is an RNA-binding protein with a mass of about 39 kDa. Msi1 is present in high levels in neural tissue and certain stem cells. It works mechanically by recognizing and binding to specific RNA sequences impacting the fate of the mRNA. Musashi 1 regulates the translation and stability of its target mRNA affecting protein synthesis at the post-transcriptional level. Its expression is not limited to neural tissues but is also detected in other tissues where stem or progenitor cells are present.
Biological function summary
In neural stem cells and other tissue types Musashi 1 plays an important role in maintaining stem cell identity. Musashi 1 forms part of a complex that controls the translation of mRNAs involved in cell fate decisions. It acts by repressing or activating the translation of key regulatory proteins that guide stem cell maintenance and differentiation processes. This protein is also involved in cellular proliferation by influencing the translation of mRNAs tied to cell cycle regulation.
Pathways
The function of Musashi 1 lies within critical signaling pathways such as Notch and Wnt. It facilitates these pathways by regulating the translation of downstream effectors which are necessary for cellular communication and differentiation. Musashi 1 associates with proteins like Numb a known Notch signaling inhibitor and Β-catenin from the Wnt pathway modulating their effects on cell cycle progression and stem cell fate.
Musashi 1's abnormal expression is often linked to conditions like glioblastoma and colorectal cancer. High levels of Musashi 1 can promote tumor growth due to its role in cell proliferation and differentiation pathways. In glioblastoma its expression relates closely with other oncogenic proteins such as Numb and Notch altering normal regulatory mechanisms. In colorectal cancer Musashi 1's interaction with Β-catenin further drives tumorigenesis highlighting its potential as a target for therapeutic interventions.