Abcam, ab52903, Anti-SMAD3 (pS423/425) + SMAD5 (pS463/465) + SMAD1 (pS463/465) + SMAD2 (pS465/467) antibody [EP823Y]

Size: 20µL / 100µL / 1mL
Anti-SMAD3 (pS423/425) + SMAD5 (pS463/465 ) + SMAD1 (pS463/465) + SMAD2 (pS465/467) antibody [EP823Y (ab52903) is a rabbit monoclonal antibody detecting Smad1 in Western Blot, IHC-P, ICC/IF, Dot Blot . Suitable for Human, Mouse . - Biophysical QC for unrivalled batch-batch consistency - Over 570 publications - Trusted since 2007
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP823Y,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:ICC/IF, Dot, WB, ChIC/CUT&RUN-seq, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody detects Smad3 phosphorylated on Serine 423 and Serine 425. This Smad3 antibody may also detect Smad1, Smad2 and Smad5 phosphorylated at the equivalent sites.

Product details:
Anti-SMAD3 (pS423/425) + SMAD5 (pS463/465 ) + SMAD1 (pS463/465) + SMAD2 (pS465/467) antibody [EP823Y] (ab52903) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ChIC/CUT&RUN-seq, Dot Blot, ICC/IF, IHC-P and WB.
Anti-SMAD3 (pS423/425) + SMAD5 (pS463/465 ) + SMAD1 (pS463/465) + SMAD2 (pS465/467) antibody [EP823Y] (ab52903) was first used in a scientific publication in 2010 and has been cited over 576 times in peer reviewed journals. It's performance in Western Blot in human and mouse samples is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-SMAD3 (pS423/425) + SMAD5 (pS463/465 ) + SMAD1 (pS463/465) + SMAD2 (pS465/467) antibody [EP823Y] (ab52903) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-SMAD3 (pS423/425) + SMAD5 (pS463/465 ) + SMAD1 (pS463/465) + SMAD2 (pS465/467) antibody [EP823Y] (ab52903) has 20 independent reviews from customers.
Anti-SMAD3 (pS423/425) + SMAD5 (pS463/465 ) + SMAD1 (pS463/465) + SMAD2 (pS465/467) antibody [EP823Y] (ab52903) specifically detects SMAD3 (pS423/425) + SMAD5 (pS463/465 ) + SMAD1 (pS463/465) + SMAD2 (pS465/467) (UniProt ID: P84022; Molecular weight: 48kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).
Conjugation-ready, carrier free format available for antibody clone EP823Y -
ab172202
Antibody clone EP823Y is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor
488, Alexa Fluor
647, Alexa Fluor
594, Alexa Fluor
568, Alexa Fluor
555, Alexa Fluor
ab310954
ab311071
ab311658
ab312931
ab313143
ab321670
Highly cited pan phospho SMAD antibody with >700 citations. Dot blot data to guarantee specificity to SMAD3 (pS423/425) + SMAD5 (pS463/465 ) + SMAD1 (pS463/465) + SMAD2 (pS465/467). Validated for CUT&RUN-seq, a key application to map protein-DNA interactions on a genome-wide scale using NGS. Phosphorylated SMAD proteins, including SMAD3 (pS423/425), SMAD5 (pS463/465), SMAD1 (pS463/465) and SMAD2 (pS465/467), play crucial roles in TGF-beta signaling pathways. These proteins are key regulators of cellular processes such as proliferation, differentiation and apoptosis. Abnormal SMAD signaling is linked to various diseases, including cancer, fibrosis and cardiovascular disorders. Monitoring these phosphorylated SMAD proteins can provide valuable insights into disease mechanisms and therapeutic targets.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please
contact us
for more information.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Smad3 also known as Mothers against decapentaplegic homolog 3 is a protein involved in the TGF-β signaling pathway. This protein weighs approximately 48 kilodaltons. It is mainly expressed in various tissues including heart lung kidney and skin. Smad3 mediates signals from the TGF-β receptors to regulate gene expression in the nucleus. This function is often studied using markers like p-Smad3 phospho-Smad3 and phospho-Smad which identify the phosphorylated forms important for its activation.
Biological function summary
Smad3 plays an important role in regulating cell proliferation differentiation and apoptosis. It is a part of the Smad complex that includes Smad2 and Smad4 which translocate to the nucleus to regulate the transcription of target genes. This activity is important for maintaining cellular functions and responding to external signals. Phosphorylation of Smad3 is an important step in its activation often studied using phospho-specific antibodies some of which are conjugated with Alexa Fluor 594 or Alexa Fluor 555 to facilitate visualization in research.
Pathways
Smad3 is an essential component of the TGF-β signaling pathway and also participates in the BMP pathway. In the TGF-β pathway it collaborates with related proteins such as Smad2 and Smad4. These proteins assemble upon receptor activation propagate the signal to the nucleus and regulate gene transcription. Smad3's function in both the TGF-β and BMP pathways demonstrates its role in controlling diverse cellular processes.
Smad3 has been linked to fibrosis and cancer. In fibrosis the overactivation of Smad3 leads to excessive extracellular matrix production contributing to tissue scarring. Cancer research highlights its role in tumorigenesis where alterations in Smad3 expression or activity affect disease progression. The connection of Smad3 with other proteins like TGF-β receptors and Smad4 in these disorders emphasizes its importance in pathological processes.