Abcam, ab52922, Anti-HLA A antibody [EP1395Y]

Size: 100µL / 1mL
Anti-HLA A antibody [EP1395Y] (ab52922) is a rabbit monoclonal antibody detecting HLA A in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF . Suitable for Human . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 90 publications - Trusted since 2007
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP1395Y,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IP, ICC/IF, WB, Flow Cyt (Intra), IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Anti-HLA A antibody [EP1395Y] (ab52922) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P, IP, WB in human samples.
Anti-HLA A antibody [EP1395Y] (ab52922) has been cited over 96 times in peer reviewed journals and is trusted by the scientific community.
Abcams high quality manufacturing and validation processes ensure Anti-HLA A antibody [EP1395Y] (ab52922) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
The specificity of Anti-HLA A antibody [EP1395Y] (ab52922) has been confirmed by testing in knockout samples.
Anti-HLA A antibody [EP1395Y] (ab52922) has 15 independent reviews from customers.
Anti-HLA A antibody [EP1395Y] (ab52922) specifically detects HLA A (UniProt ID: P04439; Molecular weight: 38kDa) and is sold in 100 uL and 1 mL selling sizes.
Conjugation-ready, carrier free format available for antibody clone EP1395Y -
ab216653
Antibody clone EP1395Y is also available pre-conjugated to a variety of labels for your convenience - HRP, Alexa Fluor® 647 (
ab199555
ab199837
HLA-A is a critical component of the immune system, presenting intracellular antigens to cytotoxic T cells, which helps in the immune response against viruses and other intracellular pathogens. HLA A has been impolicated in autoimmune diseases such as multiple sclerosis and cancer.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HLA-A also known as Human Leukocyte Antigen A is a protein that is part of the major histocompatibility complex (MHC) class I molecules. It has an approximate molecular mass of 44 kDa. HLA-A is expressed on the surface of nearly all nucleated cells and interacts with CD8+ T lymphocytes. Known for its role in immune recognition HLA-A presents endogenous peptide antigens to the T-cell receptor on cytotoxic T cells initiating immune responses to eliminate infected or malignant cells.
Biological function summary
This target functions as a component of the MHC class I molecule complex. HLA-A binds peptides derived from proteasomal degradation of intracellular proteins and presents these peptides on the cell surface. The function plays an essential role in immune surveillance and distinction between self and non-self. This antigen presentation is important for immune responses against viral infections and tumor surveillance influencing the activation and proliferation of T lymphocytes.
Pathways
HLA-A plays a significant role in the antigen processing and presentation pathway. It is closely related to the TAP (Transporter associated with Antigen Processing) proteins which transport antigenic peptides into the endoplasmic reticulum for loading onto MHC class I molecules. Additionally the target interacts with the CD8 molecule on cytotoxic T cells. This interaction is important within the immune response pathway contributing to the recognition and destruction of infected cells.
HLA-A has been associated with autoimmune diseases and transplant rejection. It plays a significant role in conditions like ankylosing spondylitis where specific HLA-A subtypes contribute to disease susceptibility. Additionally mismatches in HLA-A typing can lead to transplant rejection. In such scenarios anti-HLA antibodies can develop causing damage to transplant tissues. The complex interaction of HLA-A with related proteins such as other MHC class I molecules influences immune response outcomes in these diseases.