Abcam, ab52953, Recombinant Staphylococcus aureus Protein A

Size: 10mg
Recombinant Staphylococcus aureus Protein A is a Fragment protein in the 32 to 327 aa range with >99% purity, < 0.1 EU/mg endotoxin level and suitable for SDS-PAGE.The predicted molecular weight of ab52953 protein is 35.5 kDa.
Key facts
Purity:>99% SDS-PAGE,
Endotoxin level:< 0.1 EU/mg,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P02976,
Animal free:Yes,
Carrier free:No,
Species:Staphylococcus aureus subsp. aureus NCTC 8325,
Storage buffer:Constituents: Water

Product details:
Ensure the validity of your result using our recombinant S. aureus Protein A ab52953 to probe and detect IgG in SDS-PAGE.
Check out our protein gel staining guide for SDS-PAGE
Function
The cell wall binding region, albumin binding region and other non-specific binding regions have been eliminated from this protein to ensure the maximum specific IgG binding. The 6x His-tag can be used for affinity purification or for Protein A detection using anti-His-tag antibody. The recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies.
Protein A binds to most human and mouse IgG subclasses (e.g: human IgG1, IgG2, IgG4; mouse IgG1, IgG2a, IgG2b, IgG3).
It also binds to total IgG from cow, guinea pig, hamster, horse, pig, and rabbit.
Protein A has little affinity to chicken, goat, rat and sheep.
This product is manufactured by BioVision, an Abcam company and was previously called 6500 Protein A (Liquid form). 6500-10 is the same size as the 10 mg size of ab52953.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle