Abcam, ab53360, Anti-EB3 antibody [KT36]

Size: 100µg
Rat Monoclonal EB3 antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human, African green monkey samples. Cited in 14 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human MAPRE3.
Key facts
Host species:Rat,
Clonality:Monoclonal,
Clone number:KT36,
Isotype:IgG2a,
Carrier free:No,
Reacts with:Human, African green monkey,
Applications:Flow Cyt, WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Native Full Length Protein corresponding to Human MAPRE3.Q9UPY8

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-Preservative: 0.1% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-Up to 12 months, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
EB3 also known as End-binding protein 3 is a microtubule-associated protein with a molecular weight of approximately 32 kDa. This protein belongs to the EB family of proteins which includes EB1 and EB2. EB3 facilitates microtubule dynamics by binding to the plus ends of microtubules and playing a role in their stabilization and elongation. Researchers have observed its expression in various tissues with a notable presence in neuronal tissues where it supports complex cellular activities.
Biological function summary
EB3 interacts with other end-binding proteins as part of the plus-end tracking complex often abbreviated as +TIPs. This complex modulates the behavior of microtubules essential for processes like mitosis and cell migration. EB3’s interaction within this complex stabilizes microtubules at the growing edge important for neuronal development and synaptic function. Additionally EB3 helps in intracellular signaling through direct interaction with other microtubule-associated proteins thereby contributing to cellular structural integrity.
Pathways
EB3 plays an integral role in the Wnt signaling pathway and the microtubule polymerization regulation pathway. These pathways are important for normal cell division and signaling. In the Wnt signaling pathway EB3 interacts with proteins like APC essential for the pathway’s downstream signaling events that influence cell polarity and differentiation. Moreover its involvement in the polymerization regulation pathway helps in facilitating effective cargo transport along microtubules.
EB3 has been linked to neurodegenerative diseases such as Alzheimer’s disease and certain types of cancer. Abnormalities in EB3 expression and function can lead to disruptions in microtubule stability contributing to the pathogenesis of these conditions. EB3's connection with tau protein further highlights its role in Alzheimer’s where tau aggregation negatively affects microtubule function. In cancer alterations in EB3 expression influence tumor progression by impacting cell motility and invasiveness with implications for metastasis development.