Abcam, ab53540, Anti-ELYS antibody [2318C1a]

Size: 100µg
Mouse Monoclonal ELYS antibody. Suitable for WB and reacts with Recombinant fragment samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human AHCTF1.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:2318C1a,
Isotype:IgG1,
Carrier free:No,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human AHCTF1. The exact immunogen used to generate this antibody is proprietary information.Q8WYP5

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum, Storage buffer-pH: 7.4Preservative: 0.05% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The ELYS protein also known as MEL-28 is a critical component of the nuclear pore complexes (NPCs) and plays an important role in nuclear envelope assembly. ELYS is approximately 240 kDa in mass and is expressed in various tissues with significant presence in dividing cells. ELYS facilitates the recruitment of nucleoporins to chromatin which is necessary for the formation of NPCs during cell division.
Biological function summary
ELYS protein associates as part of the nuclear pore complex. It functions as a nucleoporin that mediates the interaction between chromatin and NPC components. This interaction is essential for the reassembly of the nuclear envelope and the reestablishment of nucleocytoplasmic transport after mitosis. ELYS acts through its AT-hook domains allowing it to bind to chromatin.
Pathways
ELYS integrates into mechanisms involving nuclear envelope reformation and nucleocytoplasmic transport pathways. It associates with key proteins in these pathways including NUP107-160 complex establishing a functional connection between chromatin and the forming NPCs. ELYS is essential for the correct localization of other nucleoporins ensuring efficient nuclear pore assembly and nuclear transport processes.
ELYS protein's dysfunction has links to cancer and neurological disorders. Defects in ELYS expression or mutations can impact correct NPC assembly affecting cellular homeostasis and leading to tumorigenesis. ELYS interaction with protein p53 plays an essential role in maintaining genomic stability which when disrupted can contribute to cancer development. Additionally changes in ELYS function have implications in neurodegenerative diseases where nuclear transport becomes compromised.