Abcam, ab54223, Anti-Ly6c antibody [ER-MP20]

Size: 100µg
Anti-Ly6c antibody [ER-MP20] (ab54223) is a rat monoclonal antibody detecting Ly6c in Flow Cytometry, IHC-Fr . Suitable for Mouse . - Over 10 publications - Trusted since 2007
Key facts
Host species:Rat,
Clonality:Monoclonal,
Clone number:ER-MP20,
Isotype:IgG2a,
Carrier free:No,
Reacts with:Mouse,
Applications:Flow Cyt, IHC-FrSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:ab54223 recognizes murine Ly-6C, a 131 amino acid ~14 kDa differentiation antigen, expressed on macrophage/dendritic cell precursors in mid-stage development (late CFU-M, monoblasts and immature monocytes), granulocytes, and on a wide range of endothelial cells and subpopulations of B- and T-lymphocytes.ab54223 is able to distinguish multiple mouse blood monocyte subsets: immature Ly-6Chi monocytes are recruited to acute peripheral inflammation and develop into Ly-6C+ exudate macrophages, whereas more mature Ly-6C-/lo monocytes are precursors for tissue macrophages and dendritic cells in steady state.ab54223 was originally described as recognizing a protein encoded by the LY6C gene. It has subsequently become apparent that the LY6C locus demonstrates polymorphism and the LY6C gene has been re-designated LY6C2. The LY6C1 gene encodes a similar protein with ~95% sequence homology to LY6C2

Product details:
This product should be stored undiluted. Shelf life - 18 months
Studies show that clone ER-MP20 can be used in conjunction with clone ER-MP12 in two colour flow cytometric analysis, to identify different stages of myeloid progenitor cells in mouse bone marrow.
Product Specifications
Anti-Ly6c antibody [ER-MP20] (ab54223) is a rat monoclonal antibody and is validated for use in Flow Cyt, IHC-Fr in mouse samples.
Anti-Ly6c antibody [ER-MP20] (ab54223) specifically detects Ly6c (UniProt ID: P0CW03; Molecular weight: 9kDa) and is sold in 100 µg selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-Ly6c antibody [ER-MP20] (ab54223) has high sensitivity and specificity.
Anti-Ly6c antibody [ER-MP20] (ab54223) has been cited over 13 times in peer reviewed journals and is trusted by the scientific community.
Related Products
Conjugation-ready, carrier free format available for antibody clone ER-MP20 -
ab236269

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant, Storage buffer-pH: 7.4Preservative: 0.09% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Ly6c also known as Ly-6C is a protein marker with a molecular weight of approximately 18 kDa. It is part of the Ly6 family of glycosylphosphatidylinositol (GPI)-anchored cell surface proteins. Ly6c is predominantly expressed in mice on the surface of certain immune cells including monocytes macrophages dendritic cells and subsets of T cells. The expression levels can vary depending on the cell type and the activation or developmental stage of the cell. Ly6c serves as a useful marker in immunological studies to distinguish between different subsets of monocytes commonly identified as Ly6c^high and Ly6c^low populations.
Biological function summary
Ly6c is involved in the differentiation and trafficking of immune cells. It is not known to be part of a larger protein complex. In the immune system the Ly6c marker is critical for distinguishing between classical and non-classical monocytes in mice. Classical monocytes expressing high levels of Ly6c are involved in rapid response to inflammatory stimuli while non-classical monocytes with lower Ly6c expression help in patrolling blood vessels. This distinction is important in understanding how the immune system responds to various infections and how the body maintains immune homeostasis.
Pathways
Ly6c plays a role in the monocyte recruitment and differentiation pathways. It is closely related to the chemokine signaling pathway influencing how immune cells migrate towards sites of inflammation or injury. The presence of the Ly6c2 protein an isoform of Ly6c helps modulate these responses by interacting with cytokines and chemokines that guide the trafficking of immune cells. The presence of Ly6c influences the activity of proteins like CCR2 which plays a significant role in chemotaxis in response to inflammation.
Ly6c is associated with inflammatory and autoimmune conditions. For example high Ly6c expression has been linked to inflammatory bowel disease (IBD) and atherosclerosis. In IBD the ability of Ly6c^high monocytes to migrate to the gut and promote inflammation is well-documented. Similarly in atherosclerosis the recruitment of Ly6c^high monocytes to blood vessels contributes to plaque formation. In these contexts proteins like MCP-1 (also known as CCL2) interact with Ly6c-expressing cells facilitating their movement and function in disease processes. Understanding the role of Ly6c in these conditions might help develop targeted therapies to mitigate chronic inflammation and related diseases.