Abcam, ab54350, Anti-SUPT4H antibody [3079C1a]

Size: 100µg
Mouse Monoclonal SUPT4H antibody 3079C1a. Suitable for WB and reacts with Recombinant fragment - Human samples. Cited in 1 publication.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:3079C1a,
Isotype:IgG2b,
Carrier free:No,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human SUPT4H1. The exact immunogen used to generate this antibody is proprietary information.P63272

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted(approximately 95%) fetal bovine serum., Storage buffer-pH: 7.4Preservative: 0.05% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-Up to 12 months, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SUPT4H also known as Suppressor of Ty 4 Homolog or SPT4 Homolog functions as part of the transcription machinery. It forms a complex known as DSIF which involves SUPT4H and a partner protein called SPT5. SUPT4H modulates RNA polymerase II (RNAPII) by forming a complex with SPT5 and facilitating transcription elongation. The molecular mass of SUPT4H is approximately 15 kDa. Expression of SUPT4H occurs broadly across various tissues indicating its essential role in general transcriptional regulation.
Biological function summary
SUPT4H plays an important role in regulating gene expression through its involvement in the DSIF complex. The DSIF complex acts as a transcription factor that associates with paused polymerase influencing transcription elongation. SUPT4H's interaction with SPT5 is important for maintaining the DSIF complex stability and function. SUPT4H and its associated complex help ensure that transcription proceeds efficiently allowing proper gene expression and cellular function.
Pathways
The DSIF complex containing SUPT4H is integral to several critical gene regulatory networks. It is involved in transcriptional pausing and control within the immediate early gene response pathway interfacing with various transcription factors and pause-release factors. Moreover the interaction with NELF (Negative Elongation Factor) provides a checkpoint control in the regulation of RNA polymerase II pausing. These interactions synchronize transcription with RNA processing and output maintaining cellular homeostasis.
SUPT4H's role in transcription regulation suggests implications in cancer and neurodegenerative disorders. Altered expression and function can disrupt normal gene expression potentially leading to oncogenesis. In the context of neurodegenerative disorders SUPT4H's regulatory activity might affect neuronal gene expression implicating it in the pathogenesis of diseases such as Alzheimer's. Its interaction with p53-responsive elements also indicates its influence on tumor suppressor gene networks linking it to cancer biology.