Abcam, ab54362, Anti-CBLB antibody [246C5a]

Size: 50µg
Anti-CBLB antibody [246C5a] (ab54362) is a mouse monoclonal antibody detecting CBLB in Western Blot, IHC-P . Suitable for Human, Mouse . - KO validated for confirmed specificity - Trusted since 2007
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:246C5a,
Isotype:IgG2a,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human CBLB. The exact immunogen used to generate this antibody is proprietary information.Q13191

Product details:
What is this antibody validated in?
Anti-CBLB antibody [246C5a] (ab54362) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Mouse samples.
What is the molecular weight of CBLB?
Anti-CBLB [246C5a] (ab54362) specifically detects a band for CBLB (UniProt: Q13191) at a molecular weight of 109kDa.
Specificity confirmed
The specificity of Anti-CBLB antibody [246C5a] (ab54362) has been confirmed by Western blot testing in CBLB Knockout HeLa cell line,
ab264711

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-ab54362 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane., Storage buffer-pH: 7.4Preservative: 0.05% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CBLB also known as Cbl-b or Casitas B-lineage lymphoma-b is an E3 ubiquitin-protein ligase with a mass of approximately 120 kDa. CBLB is required for the regulation of protein degradation and is involved in transferring ubiquitin to target proteins. It is expressed in multiple tissues including the immune cells such as T cells and B cells. This protein contains several functional domains that facilitate interaction with other proteins allowing it to influence various signaling pathways.
Biological function summary
CBLB plays a critical role in immune regulation and is part of the ubiquitin-proteasome system. As a member of the CBL protein family it interacts with several other proteins to form a complex that determines its function. This complex mediates signal transduction and maintains immune homeostasis efforting its regulatory influence over immune cell activation and avoiding excessive immune responses.
Pathways
CBLB integrates into intracellular signaling involving the T-cell receptor (TCR) and B-cell receptor (BCR) pathways. It modulates downstream signaling by finetuning protein levels and activity interfacing with related proteins such as ZAP70 and Syk. By controlling signal thresholds it ensures proper immune responses and prevents autoimmunity maintaining the balance in signal transduction processes.
Altered CBLB activity is associated with autoimmune diseases and certain cancers such as lymphoma. Its dysfunction can lead to improper regulation of immune responses allowing for unchecked immune activation or proliferation of cancer cells. CBLB interacts with other critical proteins like ITK in T cells influencing the development or progression of these diseases by affecting cell signaling dynamics. Understanding CBLB's role could support therapeutic interventions to modulate immune responses effectively.