Abcam, ab5443, Anti-PDGFR alpha + Beta (phospho Y572 + Y574) antibody

Size: 50µL
Rabbit Polyclonal PDGFR alpha phospho Y572 + Y574 antibody. Suitable for ICC, WB and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human PDGFRA phospho Y572 + Y574 aa 550-600.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WB, ICCSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human PDGFRA phospho Y572 + Y574 aa 550-600. The exact immunogen used to generate this antibody is proprietary information.P16234

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PDGFR alpha / beta. The final product is generated by affinity chromatography using a PDGFR alpha derived peptide phosphorylated at Tyrosines 572 and 574., Storage buffer-pH: 7.3Preservative: 0.05% Sodium azideConstituents: PBS, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Platelet-derived growth factor receptors alpha and beta (PDGFRα and PDGFRβ) are receptor tyrosine kinases known for their roles in cell growth and development. PDGFRα has a molecular mass of approximately 170 kDa while PDGFRβ is about 180 kDa. These receptors are found mostly on the surface of mesenchymal cells. They become activated when they bind to their specific ligands leading to receptor dimerization and autophosphorylation. This triggers intracellular signaling cascades critical for various cellular processes.
Biological function summary
PDGFRα and PDGFRβ drive cell proliferation survival and migration. They are part of signaling complexes that manage important cellular functions. These receptors control mesenchymal cell activities influencing tissue development and repair. Both receptors engage in complex interactions where they can form homodimers or heterodimers. These dimer formations allow for diversified signaling influencing a broad range of physiological functions.
Pathways
PDGFRα and PDGFRβ are essential in the MAPK/ERK and PI3K/AKT signaling pathways. These pathways regulate cell proliferation differentiation and survival. PDGFRs interact with various proteins in these pathways such as SHP2 which is an important player in the MAPK pathway. They also have dynamic crosstalk with other receptor tyrosine kinases further orchestrating cellular responses to environmental stimuli.
Dysregulation of PDGFRα and PDGFRβ has links to cancers and fibrotic diseases. In many cancers overactivation of these receptors leads to uncontrolled cellular proliferation. PDGFRβ is particularly involved in the tumor microenvironment interacting with VEGF which promotes tumor angiogenesis. In fibrotic diseases aberrant PDGFR signaling contributes to excessive tissue scarring and fibrosis due to overactive cell proliferation and migration. Understanding these pathways offers insights into therapeutic targets for these conditions.