Abcam, ab5450, Anti-GFP antibody

Size: 25µg
Anti-GFP antibody (ab5450) is a goat polyclonal antibody detecting GFP in Western Blot, IP, IHC-P, IHC- Wmt, ICC/IF, EM . - Over 240 publications - Trusted since 2004 See more GFP antibodies and assay kits
Key facts
Host species:Goat,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Applications:EM, IP, WB, IHC - Wmt, ICC/IF, IHC-P, IHC-FoFrSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Aequorea victoria GFP.P42212

Product details:
Protein A will not bind goat IgG, so use alternates (eg. protein G) in IP with this antibody.
Product Specifications
Anti-GFP antibody (ab5450) is a goat polyclonal antibody and is validated for use in EM, ICC/IF, IHC - Wmt, IHC-FoFr, IHC-P, IP, WB.
GFP antibodies are often used as loading controls in Western Blot. Anti-GFP antibody has been verified in Western Blot samples and detects a band at 27kDa Molecular weight.
Anti-GFP antibody (ab5450) specifically detects GFP (UniProt ID: P42212; Molecular weight: 27kDa) and is sold in 25 µg selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-GFP antibody (ab5450) has high sensitivity and specificity.
Anti-GFP antibody (ab5450) has been cited over 241 times in peer reviewed journals and is trusted by the scientific community.
Anti-GFP antibody (ab5450) has 13 independent reviews from customers.
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Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-This antibody is Goat anti-GFP serum (ab5449) affinity purified using a HiTrap-NHS activated sepharose column (Amersham-Pharmacia) containing covalently linked highly purified recombinant GFP. After applying the serum to the column and extensive washing, the eluted anti-GFP immunoglobulins are desalted., Storage buffer-pH: 7.4Constituents: 25% Glycerol (glycerin, glycerine), 0.79% Tris HCl, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle